Demonstration that inhibitor of rRNA synthesis in “charcoal extracts” of Xenopus embryos is artifactually produced ammonium perchlorate
Demonstration that inhibitor of rRNA synthesis in “charcoal extracts” of Xenopus embryos is artifactually produced ammonium perchlorate
复制标题
证明非洲爪蟾胚胎“木炭提取物”中的 rRNA 合成抑制剂是人工产生的高氯酸铵
DOI：
10.1016/0012-1606(85)90141-1
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发表时间：
1985
影响因子：
2.7
通讯作者：
K. Yamana
中科院分区：
文献类型：
--
作者：
K. Shiokawa;Y. Kawazoe;K. Yamana
用木炭柱部分纯化的xenopuscleavage胚胎的高氯酸提取物选择性地抑制xenopusneurula细胞中的rRNA合成。长期以来，人们一直认为活性物质是与核苷酸有关的物质，因为它似乎与木炭柱结合。然而，活性物质被证明是高氯酸铵，当解理胚胎的高氯酸提取物用氨醇从木炭柱洗脱时，人工产生了高氯酸铵。高氯酸盐的抑制活性与铵离子无关，而与高氯酸盐无关。
A perchloric acid extract ofXenopuscleavage embryos partially purified with a charcoal column selectively inhibits rRNA synthesis inXenopusneurula cells. The active substance has long been assumed to be nucleotide-related material, because it appeared to bind to a charcoal column. However, the active substance was proved to be ammonium perchlorate that was produced artifactually when a perchloric acid extract of cleavage embryos was eluted from a charcoal column with ammonia-alcohol. Ammonium ion, but not perchlorate ion, was responsible for the inhibitory activity.