Integron-encoded Intl integrases preferentially recognize the adjacent cognate attl site in recombination with a 59-be site
Integron-encoded Intl integrases preferentially recognize the adjacent cognate attl site in recombination with a 59-be site
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DOI：
10.1046/j.1365-2958.2002.03260.x
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发表时间：
2002-12-01
影响因子：
3.6
通讯作者：
Hall, RM
中科院分区：
文献类型：
--
作者：
Collis, CM;Kim, MJ;Hall, RM
整合子有能力捕获被称为基因盒的小的可移动的元件，这个反应是由整合子编码的整合酶催化的。内含子整合酶在酪氨酸重组酶超家族中形成了一个独特的家族，并包括一个保守的特征附加域。两种不同的Inti酶被用来检测它们在整合和切除分析中识别异源Atti位点的能力。IntI1和IntI3有59%的同源性，并催化盒相关的59-Be位点和同源attI1或attI3位点之间的整合和激活重组。涉及59-BE和非同源Atti位点的整合重组事件极少被检测到，IntI1的attI2和attI3或IntI3的attI1和attI2。在盒切除实验中，非同源的attI3位点被IntI1识别，但attI1不能很好地被IntI3识别。纯化的IntI1和IntI3蛋白仅与它们的同源ATT1位点强烈结合。
Integrons have the capacity to capture small mobile elements known as gene cassettes, and this reaction is catalysed by integron-encoded IntI integrases. IntI integrases form a distinct family within the tyrosine recombinase superfamily and include a characteristic additional domain that is well conserved. Two different IntI enzymes were used to examine their ability to recognize heterologous attI sites in both integration and excision assays. IntI1 and IntI3 are 59% identical and catalyse both integrative and excisive recombination between a cassette-associated 59-be site and the cognate attI1 or attI3 site. Integrative recombination events involving a 59-be and a non-cognate attI site, attI2 and attI3 for IntI1 or attI1 and attI2 for IntI3, were detected extremely rarely. In cassette excision assays, the non-cognate attI3 site was recognized by IntI1, but attI1 was not well recognized by IntI3. The purified IntI1 and IntI3 proteins bound strongly only to their cognate attl site.