Enhanced chemiluminescent resonance energy transfer in hollow calcium phosphate nanoreactors and the detection of hydrogen peroxide
Enhanced chemiluminescent resonance energy transfer in hollow calcium phosphate nanoreactors and the detection of hydrogen peroxide
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DOI：
10.1088/0957-4484/18/29/295707
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发表时间：
2007-07
期刊：
影响因子：
3.5
通讯作者：
P. Wingert;Hiroshi Mizukami;Agnes Ostafin
中科院分区：
文献类型：
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作者：
P. Wingert;Hiroshi Mizukami;Agnes Ostafin
描述了化学发光纳米反应器（纳米-CRET），其在与过氧化氢反应时表现出异常有效的化学发光共振能量转移到共包封的荧光团。纳米颗粒的平均尺寸为150 ± 20 nm，由磷酸钙壳包围中空核组成，中空核填充有含有鲁米诺、血红素和荧光素的溶液。稳态和停流荧光光谱被用来表征其过氧化氢依赖的排放。结果表明，在化学激发的鲁米诺中间体和荧光素染料之间的共振能量转移效率的显着改善，在溶液中，当鲁米诺和荧光素共封装内纳米-CRET。这种纳米CRET颗粒可以提高许多生命科学和法医学应用中H2 O2传感的容易性和准确性，特别是在试剂降解或其他分子对荧光团发射的猝灭阻碍定量分析的情况下。
Chemiluminescent nanoreactors (nano-CRET) that exhibit unusually efficient chemiluminescence resonance energy transfer to a co-encapsulated fluorophore when reacted with hydrogen peroxide are described. The nanoparticles have a mean size of 150 ± 20 nm and consist of a calcium phosphate shell surrounding a hollow core that is filled with a solution containing luminol, haematin and fluorescein. Steady state and stopped-flow fluorescence spectroscopy were used to characterize their H2O2-dependent emissions. The results demonstrated a significant improvement in resonance energy transfer efficiency between the chemically excited luminol intermediate and fluorescein dye over that in solution when luminol and fluorescein were co-encapsulated inside nano-CRET. Such nano-CRET particles can improve the ease and accuracy of H2O2 sensing in many life science and forensics applications, particularly where reagent degradation or quenching of the chemiluminophore emission by other molecules impedes quantitative analysis.