Fermentation of corn fiber hydrolysate to lactic acid by the moderate thermophile Bacillus coagulans

Fermentation of corn fiber hydrolysate to lactic acid by the moderate thermophile Bacillus coagulans
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DOI:
10.1007/s10529-010-0222-z
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发表时间:
2010-06-01
影响因子:
2.7
通讯作者:
Rich, Joseph O.
Rich, Joseph O.
中科院分区:
工程技术4区
文献类型:
--
作者:
Bischoff, Kenneth M.;Liu, Siqing;Rich, Joseph O.

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从堆肥牛粪中分离到一株凝结芽孢杆菌,它能在50摄氏度的温度下将葡萄糖、木糖和阿拉伯糖的混合糖转化为L-乳酸,产率为85%。该菌株耐醛生长抑制剂的浓度分别为2.5g/L、2.5g 5-羟甲基呋喃/L、2.5g香兰素/L和1.2g对羟基苯甲醛/L。在同步糖化发酵过程中,该菌株在50A℃下72h内将100g玉米纤维/L的稀酸水解物转化为39g乳酸/L,该菌株具有耐抑制性和充分利用戊糖的能力,有可能作为农业残渣转化为有价值的化学品的生物催化剂。
A strain of Bacillus coagulans that converted mixed sugars of glucose, xylose, and arabinose to l-lactic acid with 85% yield at 50A degrees C was isolated from composted dairy manure. The strain was tolerant to aldehyde growth inhibitors at 2.5 g furfural/l, 2.5 g 5-hydroxymethylfurfural/l, 2.5 g vanillin/l, and 1.2 g p-hydroxybenzaldehyde/l. In a simultaneous saccharification and fermentation process, the strain converted a dilute-acid hydrolysate of 100 g corn fiber/l to 39 g lactic acid/l in 72 h at 50A degrees C. Because of its inhibitor tolerance and ability to fully utilize pentose sugars, this strain has potential to be developed as a biocatalyst for the conversion of agricultural residues into valuable chemicals.