Cellular mRNA expressions of anti-oxidant factors in the blood of preeclamptic women
Cellular mRNA expressions of anti-oxidant factors in the blood of preeclamptic women
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DOI：
10.1002/pd.2278
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发表时间：
2009-07-01
影响因子：
3
通讯作者：
Okai, Takashi
中科院分区：
文献类型：
--
作者：
Nakamura, Masamitsu;Sekizawa, Akihiko;Okai, Takashi
目的探讨子痫前期孕妇外周血中氧化应激相关基因mRNA表达的变化。对细胞RNA进行逆转录聚合酶链反应（PCR）测定，以检查在妊娠35-41周期间先兆子痫妇女（n = 24）和对照受试者（n = 24）中的mRNA分布。结果子痫前期患者与正常对照组相比，氧化应激相关基因的表达差异有统计学意义。先兆子痫组血红素氧合酶（HO）-1的值较低。HO-2、过氧化氢酶和超氧化物歧化酶（SOD）。HO-1 HO-2和过氧化氢酶水平与蛋白尿显著相关，HO-2水平与收缩压显著相关。HO-2、SOD和过氧化氢酶存在于先兆子痫患者血液的细胞成分中。这些值与先兆子痫的严重程度相关。这些发现表明，增强的氧化应激和抗氧化酶数量的减少可能与先兆子痫有关。版权所有（C）2009年约翰威利父子。公司
Objective To assess the alterations of mRNA expressions associated with oxidative stress in file cellular component of blood front pregnant women with pre-eclampsia.Methods Peripheral blood samples were obtained from pregnant women With and without pre-eclampsia. Cellular RNA Was subjected to a reverse transcription polymerase chain reaction (PCR) assay in order to examine the mRNA distribution among women with pre-eclampsia (n = 24) and control subjects (n = 24) during 35-41 weeks of gestation. The data were analyzed by non-parametric statistics.Results Significant differences between the pre-eclampsia subjects and the controls were observed in the gene expressions associated with oxidative stress. Lower values in the pre-eclampsia group were found for heme oxygenase (HO)-1. HO-2, catalase and superoxide dismutase (SOD). The HO-1. HO-2 and the catalase levels significantly correlated with proteinuria, and the HO-2 level with systolic blood pressure.Conclusion Significantly lower, concentrations of HO-1. HO-2, SOD and catalase are found in the cellular component of blood from pre-eclamptic patients. The values correlate with the severity of, pre-eclampsia. These findings indicate that enhanced oxidative stress mid a decrease in the number of anti-oxidant enzymes may be associated with pre-eclampsia. Copyright (C) 2009 John Wiley & Sons. Ltd.