SIMULTANEOUS DETERMINATION OF ALPHA-TOCOPHEROL AND RETINOL IN PLASMA OR RED-CELLS BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY
SIMULTANEOUS DETERMINATION OF ALPHA-TOCOPHEROL AND RETINOL IN PLASMA OR RED-CELLS BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY
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DOI：
10.1093/ajcn/32.10.2143
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发表时间：
1979-01-01
影响因子：
7.1
通讯作者：
CATIGNANI, GL
中科院分区：
文献类型：
--
作者：
BIERI, JG;TOLLIVER, TJ;CATIGNANI, GL
描述了用于同时测定[人] α-生育酚（维生素E）和视黄醇（维生素A），以及α-维生素E在红细胞中，因为细胞不含视黄醇。来自0.1ml血浆或0.125ml红细胞的总脂质提取物，其含有α-将生育酚乙酸酯和视黄基乙酸酯注射到具有用甲醇水展开的反相柱的高压液相色谱仪上。使用具有280 nm滤光片的UV检测器。色谱图在8分钟内完成，α-生育酚和视黄醇通过峰高比法定量。与血浆和红细胞的结果比较，这些维生素与传统方法非常一致。该程序对临床研究和营养调查特别有用。
A rapid microprocedure is described for the simultaneous determination of [human] .alpha.-tocopherol (vitamin E) and retinol (vitamin A) in plasma and of .alpha.-tocopherol in red cells since cells do not contain retinol. A total lipid extract from 0.1 ml plasma or 0.125 ml red cells containing internal standards of .alpha.-tocopheryl acetate and retinyl acetate is injected onto a high pressure liquid chromatograph with a reverse phase column developed with methanol water. An UV detector with 280 nm filter is used. The chromatogram is complete in 8 min and the .alpha.-tocopherol and retinol are quantitated by the peak height ratio method. Comparison of results with plasma and red cells gave excellent agreement with conventional methods for these vitamins. The procedure should be particularly useful for clinical studies and nutrition surveys.