Deoxyribonucleic acid relatedness among Mycobacterium leprae, Mycobacterium lepraemurium, and selected bacteria by dot blot and spectrophotometric deoxyribonucleic acid hybridization assays
Deoxyribonucleic acid relatedness among Mycobacterium leprae, Mycobacterium lepraemurium, and selected bacteria by dot blot and spectrophotometric deoxyribonucleic acid hybridization assays
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通过斑点印迹和分光光度脱氧核糖核酸杂交测定麻风分枝杆菌、麻风分枝杆菌和选定细菌之间的脱氧核糖核酸相关性
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
T. Imaeda
中科院分区：
文献类型：
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作者：
R. Athwal;S. Deo;T. Imaeda
用斑点杂交和分光光度脱氧核糖核酸杂交方法研究了麻风分枝杆菌或麻风分枝杆菌与其他选定细菌脱氧核糖核酸的相关性。除麻风杆菌与两株棒状杆菌的亲缘关系值不同外，两种方法所得结果基本一致。在所研究的分枝杆菌物种中，从螳螂和一只患有麻风样疾病的猕猴中分离出的抗酸微生物显示，100%与实验中在螳螂中生长的麻风杆菌有关，这表明它们是共同的起源。在这项研究中，我们论证了斑点杂交技术作为分枝杆菌分类筛选方法的有效性。
Deoxyribonucleic acid relatedness between Mycobacterium leprae or Mycobacterium lepraemurium and other selected bacteria was studied by both dot blot and spectrophotometric deoxyribonucleic acid hybridization assays. The results obtained by the two methods were similar, except for the relatedness values between M. leprae and two corynebacterial strains. Among the mycobacterial species examined, acid-fast organisms isolated from armadillos and a mangabey monkey with leprosy-like disease showed 100% relatedness with M. leprae grown experimentally in armadillos, suggesting their common origin. In this study we demonstrate the usefulness of the dot blot hybridization technique as a screening method for mycobacterial taxonomy.