Inhibition by L-arginine and spermidine of hemoglobin glycation and lipid peroxidation in rats with induced diabetes
Inhibition by L-arginine and spermidine of hemoglobin glycation and lipid peroxidation in rats with induced diabetes
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DOI：
10.1016/j.biopha.2005.08.004
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发表时间：
2006-01-01
影响因子：
7.5
通讯作者：
Balderas, FL
中科院分区：
文献类型：
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作者：
Méndez, JD;Balderas, FL
本文研究了L-精氨酸和亚精胺对正常和糖尿病大鼠血红蛋白糖化和血清脂质过氧化的影响。在20天内对5组40只大鼠进行了研究，并与对照组（I组）进行了比较，对照组由未用L-精氨酸或亚精胺处理的正常大鼠（N = 6）组成。II组，糖尿病大鼠（四氧嘧啶120 mg/kg，第0天腹腔注射，和四氧嘧啶60 mg/kg，第10天腹腔注射）被视为糖尿病对照。组III，用10 MM L-精氨酸（i.p.）处理的糖尿病大鼠。组IV，用10 μ M亚精胺（i.p.）处理的糖尿病大鼠。V组，正常大鼠腹腔注射10 MM L-精氨酸。VI组，正常大鼠腹腔注射10 μ M亚精胺。将每组大鼠分成亚组，每组4只。麻醉大鼠，从主动脉采血，测定葡萄糖、甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白和高密度脂蛋白（LDL和HDL）、糖化血红蛋白（HbA（1C））和硫代巴比妥酸反应物质（TBARS）。我们观察到，本研究中使用的四氧嘧啶浓度在96小时内再现了疾病的临床表现，包括高血糖症（从116 +/-7 mg/dl到43 +/-80 mg/dl）。结果，TG、TC、LDL、TBARS和HbA（1C）水平升高，而HDL降低。HbA（1C）浓度与TBARS浓度呈显著相关。L-精氨酸和亚精胺注射倾向于从24小时开始使血糖正常化，类似地，高脂血症、TBARS和HbA（1C）也趋于正常化。从这些结果中，我们得出结论，L-精氨酸和亚精胺产生抑制作用的血红蛋白糖化和脂质过氧化反应在体内，这可能是有关预防糖尿病并发症。(c)2005年，Elsevier SAS。All rights reserved.
The effect Of L-arginine and spermidine on hemoglobin glycation and lipid peroxidation in serum of normal and diabetic rats was studied. Five groups of 40 rats were studied during 20 days and compared with a control group (Group I) that consisted of normal rats (N = 6) not treated with L-arginine or spermidine. Group II, diabetic rats (alloxan 120 mg/kg, i.p. at the day 0 and alloxan 60 mg/kg, i.p. at the day 10) were considered as diabetic control. Group III, diabetic rats treated with 10 MM L-arginine (i.p.). Group IV, diabetic rats treated with 10 mu M spermidine (i.p.). Group V, normal rats treated with 10 MM L-arginine (i.p.). Group VI, normal rats treated with 10 mu M spermidine (i.p.). The rats of each group were divided in subgroups of four each. Rats were anesthetized and blood was taken from aorta to determine glucose, triglycerides (TGs), total cholesterol (TC), low- and high-density lipoproteins (LDL and HDL), glycated hemoglobin (HbA(1C)), and thiobarbituric acid-reactive substances (TBARS). We observed that the alloxan concentrations used in this study reproduced the clinical manifestations of disease including hyperglycemia (from 116 +/- 7 mg/dl to 43 +/- 80 mg/dl) in 96 hours. As a consequence the levels of TGs, TC, LDL, TBARS, and HbA(1C) were increased, whereas HDL diminished. HbA(1C) concentration was significantly correlated with the concentration of TBARS. The L-arginine and spermidine injection tended to normalize the glycemia from 24 hours, similarly, hyperlipidemia, TBARS, and HbA(1C). From these results, we conclude that L-arginine and spermidine exerted an inhibitory effect of hemoglobin glycation and lipid peroxidation in vivo, which may be relevant in preventing diabetic complications. (c) 2005 Elsevier SAS. All rights reserved.