The effect of temperature on cellular reactions of Crassostrea virginica to the injection of avian erythrocytes.
The effect of temperature on cellular reactions of Crassostrea virginica to the injection of avian erythrocytes.
复制标题
温度对弗吉尼亚巨牡蛎注射禽红细胞反应的影响。
DOI：
10.1016/0022-2011(74)90068-8
复制
发表时间：
1974
影响因子：
3.4
通讯作者：
J. S. Feng
中科院分区：
文献类型：
--
作者：
S. Y. Feng;J. S. Feng
在15-19°C下，将鸡红细胞（RBC）心内注射到牡蛎Crassostrea virginica中，引起白细胞的摄入，消化和细胞清除。早在注射后1小时，也可检测到成纤维细胞样细胞和特征性细胞聚集体的快速反应。虽然前者进行了一次不成功的尝试，将载有红细胞的白细胞包封在血管内，但后者细胞类型的功能尚未确定。在流动海水条件下处理牡蛎中的红细胞的过程与在静水条件下观察到的牡蛎中的过程没有明显不同。在先前用红细胞致敏的牡蛎中，对挑战注射的反应与第一次注射后观察到的反应相似，并且没有迹象表明白细胞的活性增强。然而，已证明牡蛎白细胞在至少22天内保持功能活性。有证据表明，在第二次注射红细胞后，特征性细胞聚集体的数量显著增加，尽管这种反应的特异性尚待确定。在6°C下，尽管血细胞的吞噬作用相当广泛，但白细胞表现出相当低的消化和迁移活性，这导致血细胞的有效处置要低得多。初步观察还显示，在此温度下接种后，牡蛎白细胞可能粘附到血管壁上。
Intracardial injection of chicken red blood cells (RBC) into the oyster,Crassostrea virginica, at 15–19°C elicited ingestion, digestion, and removal of the cells by the leukocytes. A rapid response of fibroblastlike cells and characteristic cell aggregates were also detected as early as1hr post-injection. While the former was engaged in an unsuccessful attempt to encapsulate red blood cell laden leukocytes intravascularly, the function of the latter cell type was undetermined. The process of disposing the red blood cells in the oysters under running seawater conditions was not noticeably different from that observed in the oysters under standing water conditions.In oysters previously sensitized with red blood cells, response to a challenge injection was similar to that observed following the first injection, and there was no indication that the activities of the leukocytes were enhanced. However, it was demonstrated that oyster leukocytes remained functionally viable for at least 22 days. There is evidence indicating that the number of the characteristic cell aggregates increases considerably after a secondary injection of red blood cells, although the specificity of this response is yet to be determined.At 6°C, although phagocytosis of the blood cells occurred quite extensively, leukocytes exhibited considerably low digestive and migratory activity which resulted in far less effective disposal of the blood cells. Preliminary observations also revealed possible adhesion of oyster leukocytes to the wall of blood vessels following the inoculation at this temperature.