SIMPLE AND RAPID METHOD FOR ISOLATING LARGE PLASMID DNA FROM LACTIC STREPTOCOCCI
SIMPLE AND RAPID METHOD FOR ISOLATING LARGE PLASMID DNA FROM LACTIC STREPTOCOCCI
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DOI：
10.1128/aem.46.3.549-552.1983
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发表时间：
1983-01-01
影响因子：
4.4
通讯作者：
MCKAY, LL
中科院分区：
文献类型：
--
作者：
ANDERSON, DG;MCKAY, LL
本文描述了一种从乳酸链球菌中快速分离大于30兆道尔顿的质粒DNA的方法。该方案可用于制备规模，以分离限制性分析、克隆或转化实验所需的足够量的质粒DNA。一个规模缩小的协议是非常有用的快速筛选链球菌菌株的质粒含量。使用这种方法，观察到以前未检测到的大质粒。
A procedure for the rapid isolation of plasmid DNA larger than 30 megadaltons from lactic streptococci is described. This protocol can be used on a preparative scale to isolate sufficient quantities of plasmid DNA required for restriction analysis, cloning or transformation experiments. A scaled-down protocol is very useful for rapidly screening the plasmid content of streptococcal strains. With this methodology, previously undetected large plasmids were observed.