Unequal variation in the two genome parts of tobraviruses and evidence for the existence of three separate viruses.
Unequal variation in the two genome parts of tobraviruses and evidence for the existence of three separate viruses.
复制标题
烟草病毒两个基因组部分的不平等变异以及存在三种不同病毒的证据。
DOI：
10.1099/0022-1317-66-1-171
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
B. Harrison
中科院分区：
文献类型：
--
作者：
D. Robinson;B. Harrison
摘要
利用互补DNA拷贝进行的杂交实验表明，14个烟草花叶病毒分离物可分为3个组，分别为10个烟草拨浪病毒(TRV)血清I-II、1个TRV血清III和3个豌豆早褐变病毒(PEBV)。来自每个基团成员的RNA与同一基团其他成员的RNA具有很大的核苷酸序列同源性，但与来自另外两个基团成员的RNA几乎或没有同源性。10个TRV血清型I-II型分离株在其RNA-1物种上具有广泛的序列，但在其RNA-2物种和其颗粒的抗原性方面表现出很大的多样性。然而，在血清学上密切相关的分离株的RNA-2序列之间发现了同源性。由于CAM株(TRV血清型III)与PEBV一样，在核苷酸序列、抗原特异性和生物学特性上都与TRV血清型I-II型分离株不同，因此它现在被认为是一个独立的病毒，建议重新命名为辣椒环斑病毒。
Summary
Hybridization experiments using complementary DNA copies showed that 14 tobravirus isolates could be divided into three groups, comprising ten of tobacco rattle virus (TRV) serotype I-II, one of TRV serotype III, and three of pea early-browning virus (PEBV), respectively. RNA from members of each group has much nucleotide sequence homology with RNA of other members of the same group but little or none with RNA from members of the two other groups. The ten TRV serotype I-II isolates share extensive sequences in their RNA-1 species, but show much diversity in their RNA-2 species and in the antigenic relatedness of their particles. However, homology was found between the RNA-2 sequences of isolates that are serologically closely related. Because strain CAM (TRV serotype III) is as different from isolates of TRV serotype I-II in nucleotide sequence, antigenic specificity and biological properties as is PEBV, it is now considered to be a separate virus and a return to its original name, pepper ringspot virus, is recommended.