Proteomics: quantitative and physical mapping of cellular proteins
Proteomics: quantitative and physical mapping of cellular proteins
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DOI：
10.1016/s0167-7799(98)01245-1
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发表时间：
1999-03-01
影响因子：
17.3
通讯作者：
Weir, MP
中科院分区：
文献类型：
--
作者：
Blackstock, WP;Weir, MP
基因组测序提供了大量关于预测基因产物（主要是蛋白质）的信息，但其中大多数没有已知的功能。二维凝胶电泳和质谱分析，加上在蛋白质和EST数据库中的搜索，改变了蛋白质鉴定过程。蛋白质组是基因组表达的蛋白质的补充，蛋白质组学是蛋白质水平上的功能基因组学。蛋白质组学可以分为表达蛋白质组学，研究蛋白质表达的整体变化，和细胞图谱蛋白质组学，通过分离蛋白质复合物系统研究蛋白质-蛋白质相互作用。
Genome sequencing provides a wealth of information on predicted gene products (mostly proteins), but the majority of these have no known function. Two-dimensional gel electrophoresis and mass spectrometry have, coupled with searches in protein and EST databases, transformed the protein-identification process. The proteome is the expressed protein complement of a genome and proteomics is functional genomics at the protein level. Proteomics can be divided into expression proteomics, the study of global changes in protein expression, and cell-map proteomics, the systematic study of protein-protein interactions through the isolation of protein complexes.