Si3N4-bioglass composites stimulate the proliferation of MG63 osteoblast-like cells and support the osteogenic differentiation of human bone marrow cells
Si3N4-bioglass composites stimulate the proliferation of MG63 osteoblast-like cells and support the osteogenic differentiation of human bone marrow cells
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DOI：
10.1016/s0142-9612(02)00249-1
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发表时间：
2002-12-01
期刊：
影响因子：
14
通讯作者：
Fernandes, MH
中科院分区：
文献类型：
--
作者：
Amaral, M;Costa, MA;Fernandes, MH
新型si3n4 -生物玻璃复合材料（重量比70-30%）的体外骨相容性改善了力学性能(断裂韧性= 4.4 MPa m(1/2)；抗弯强度= 383±47 MPa)。复合材料样品在培养基中浸泡30分钟至7天，导致蛋白质快速吸附到表面，并且生物玻璃的晶间相溶解（时间依赖过程），形成不同大小的空腔。在5天的培养期间，“接收”和预处理的材料样品在MG63成骨样细胞的增殖方面表现出相似的行为。与在标准塑料培养板上进行的培养相比，种子材料显示出更高的细胞生长速度。为了评估复合材料的成骨潜力，将“接收”材料样品与人骨髓细胞一起播种，并在有利于成骨表型发育的实验条件下培养35天。细胞粘附过程与对照培养相似。细胞成功地适应了表面的不规则性，并能够向腔内生长；此外，成骨分化伴随着大量细胞介导的矿化沉积的形成。结果表明，这种si3n4 -生物玻璃复合材料似乎是高应力医疗应用的有希望的候选者。(C) 2002 Elsevier Science Ltd.版权所有。
The in vitro osteocompatibility of a novel Si3N4-bioglass composite (70-30% weight proportion) with improved mechanical properties (fracture toughness = 4.4 MPa m(1/2); bending strength = 383 +/- 47 MPa) is reported. Immersion of the composite samples in culture medium (30 min to 7 days) resulted in rapid protein adsorption to the surface and, also, dissolution of the intergranular phase of bioglass (time-dependent process) with the formation of different size cavities. "As-received" and pre-treated material samples presented a similar behaviour concerning the proliferation of MG63 osteoblast-like cells, evaluated during a 5-day culture period. Seeded materials showed a higher cell growth rate as compared to cultures performed on the standard plastic culture plates. To assess the osteogenic potential of the composite, "as-received" material samples were seeded with human bone marrow cells and cultured for 35 days in experimental conditions that favour the development of the osteoblastic phenotype. The cell adhesion process was similar to that observed in control cultures. Cells successfully adapted to the irregularities of the surface and were able to grow towards inside the cavities; in addition, osteogenic differentiation occurred with the formation of abundant cell-mediated mineralised deposits. Results suggest that this Si3N4-bioglass composite seems to be a promising candidate for high-stress medical applications. (C) 2002 Elsevier Science Ltd. All rights reserved.