Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA
Trm11p and Trm112p are both required for the formation of 2-methylguanosine at position 10 in yeast tRNA
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DOI：
10.1128/mcb.25.11.4359-4370.2005
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发表时间：
2005-06-01
影响因子：
5.3
通讯作者：
Lapeyre, B
中科院分区：
文献类型：
--
作者：
Purushothaman, SK;Bujnicki, JM;Lapeyre, B
N-2-单甲基鸟苷-10 （m(2) G10）和n - 2，n -2-二甲基鸟苷-26 (m（2) (2)G26）是仅有的两种在几乎所有古生菌和真核生物的tRNA中检测到的鸟苷修饰，但在细菌中没有。在酿酒酵母中，m(2) (2)G26的形成是由Trm1p催化的，我们在此报告了在酵母tRNA中催化m(2) G10形成的酶活性的鉴定。它由至少两个在体内相关的亚基组成：Trm11p （Yol124c）是催化亚基，Trm112p （Ynr046w）是一种推定的锌结合蛋白。虽然TRM11的缺失在实验室条件下没有可检测到的表型，但TRM112的缺失会导致严重的生长缺陷，这表明它在细胞中具有额外的功能。事实上，Trm112p与至少四种蛋白质相关：两个tRNA甲基转移酶（Trm9p和Trm11p），一个推测的蛋白质甲基转移酶（Mtc6p/Ydr140w），一个具有罗斯曼折叠脱氢酶结构域的蛋白质（Lys9p/Ynr050c）。此外，TRM11与TRM1相互作用，这表明m(2) G10和m(2) 2G26的缺失会影响tRNA的代谢或功能。
N-2-Monomethylguanosine-10 (m(2) G10) and N-2,N-2-dimethylguanosine-26 (m(2) (2)G26) are the only two guanosine modifications that have been detected in tRNA from nearly all archaea and eukaryotes but not in bacteria. In Saccharomyces cerevisiae, formation Of m(2) (2)G26 is catalyzed by Trm1p, and we report here the identification of the enzymatic activity that catalyzes the formation Of m(2) G10 in yeast tRNA. It is composed of at least two subunits that are associated in vivo: Trm11p (Yol124c), which is the catalytic subunit, and Trm112p (Ynr046w), a putative zinc-binding protein. While deletion of TRM11 has no detectable phenotype under laboratory conditions, deletion of TRM112 leads to a severe growth defect, suggesting that it has additional functions in the cell. Indeed, Trm112p is associated with at least four proteins: two tRNA methyltransferases (Trm9p and Trm11p), one putative protein methyltransferase (Mtc6p/Ydr140w), and one protein with a Rossmann fold dehydrogenase domain (Lys9p/Ynr050c). In addition, TRM11 interacts genetically with TRM1, thus suggesting that the absence Of m(2) G10 and m(2) 2G26 affects tRNA metabolism or functioning.