A SPLIT ZINC-FINGER PROTEIN IS REQUIRED FOR NORMAL YEAST GROWTH
A SPLIT ZINC-FINGER PROTEIN IS REQUIRED FOR NORMAL YEAST GROWTH
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DOI：
10.1016/0378-1119(91)90302-r
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发表时间：
1991-10-30
期刊：
影响因子：
3.5
通讯作者：
SILVER, P
中科院分区：
文献类型：
--
作者：
BLUMBERG, H;SILVER, P
我们已经确定了一个基因，当存在于多个副本，部分抑制核蛋白定位在酿酒酵母。 该基因编码的蛋白质是DNA结合蛋白的Cys2His2锌指家族的独特成员。 它被指定为裂指蛋白的SFP1，因为它的两个锌指结构域由40个氨基酸（aa）彼此分开，而不是Cys2His2蛋白通常的7或8个aa的间距。 SFP1基因的破坏导致细胞生长缓慢，细胞具有多个有核芽。
We have identified a gene that, when present in multiple copies, partially inhibits nuclear protein localization in Saccharomyces cerevisiae. This gene encodes a protein that is a unique member of the Cys2His2 zinc-finger family of DNA-binding proteins. It is designated SFP1 for split finger protein because its two zinc-finger domains are separated from one another by 40 amino acids (aa) as opposed to the usual spacing of 7 or 8 aa for Cys2His2 proteins. Disruption of the SFP1 gene results in slow cell growth, with cells having multiple, nucleated buds.