CELL CYCLE-SPECIFIC GLUCOCORTICOID GROWTH-REGULATION OF A HUMAN CELL-LINE (NHIK 3025)
CELL CYCLE-SPECIFIC GLUCOCORTICOID GROWTH-REGULATION OF A HUMAN CELL-LINE (NHIK 3025)
复制标题
DOI：
10.1002/jcp.1041090315
复制
发表时间：
1981-01-01
影响因子：
5.6
通讯作者：
EIKNES, KB
中科院分区：
文献类型：
--
作者：
BAKKE, O;EIKNES, KB
据报道，人类细胞系NHIK 3025具有特异性的细胞质糖皮质激素受体。当这些细胞暴露于糖皮质激素时，细胞周期时间延长。细胞，同步的有丝分裂选择，受到合成糖皮质激素地塞米松在整个细胞周期。只有G1期前半期暴露的细胞周期时间延长。大部分延长也发生在G1期。地塞米松生长抑制是可逆的，并且只能在细胞暴露于类固醇的细胞周期中检测到。用流式细胞术记录非同步细胞在类固醇暴露后不同时间的dna直方图。这些直方图还显示了地塞米松暴露后G1期的敏感性和G1期的延长。这些细胞在细胞周期的g1期中期明显存在地塞米松敏感限制点。
The human cell line NHIK 3025 reportedly has a specific cytoplasmic glucocorticoid receptor. When these cells were exposed to glucocorticoids, the cell cycle time was prolonged. Cells, synchronized by mitotic selection, were subjected to the synthetic glucocorticoid dexamethasone throughout the cell cycle. Only cells exposed in the 1st half of G1 phase had a lengthened cell cycle time. Most of the prolongation was also located within the G1 phase. The dexamethasone growth inhibition was reversible and could be detected only in the cell cycle where the cells were exposed to the steroid. DNA-histograms of asynchronous cells were recorded by flowcytometry at various times after steroid exposure. These histograms also showed G1 phase sensitivity and G1 phase prolongation after exposure to dexamethasone. These cells apparently have a dexamethasone-sensitive restriction point in mid-G1 phase of the cell cycle.