Xenomonitoring of Culex quinquefasciatus mosquitoes as a guide for detecting the presence or absence of lymphatic filariasis:: a preliminary protocol for mosquito sampling
Xenomonitoring of Culex quinquefasciatus mosquitoes as a guide for detecting the presence or absence of lymphatic filariasis:: a preliminary protocol for mosquito sampling
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DOI：
10.1179/000349802125002365
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发表时间：
2002-12-01
影响因子：
--
通讯作者：
Ottesen, EA
中科院分区：
文献类型：
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作者：
Chadee, DD;Williams, SA;Ottesen, EA
本文介绍了一种采集静置、充血致倦库蚊（Culex quinquefasciatus Say）并进行微丝蚴检查的方法，以检测当地是否发生淋巴丝虫病（LF）。该议定书首先在西印度群岛特立尼达的一项试点研究中实施。为了收集流行率数据，这种异种监测是使用人类诱饵的适当替代方法，人类诱饵在伦理上是有问题的。使用电动和口腔吸引器收集室内或室外的休眠蚊子。研究人员发现，用一束椰子叶的中脉制成的“椰子扫帚”可以有效地驱赶睡在卧室内难以到达的地方的蚊子。介绍了该战略的基本原理和五步方法，即通知住户、绘图、准备设备、收集蚊子和实验室处理。试点研究的数据表明，这种异种监测方案可能适用于全世界，尽管需要考虑到所涉病媒物种及其生态和休眠行为的变化进行修改。
A protocol for the collection of resting, blood-engorged Culex quinquefasciatus Say and their examination for microfilariae has been developed as a way of detecting whether lymphatic filariasis (LF) occurs in a particular locality. The protocol was first implemented in a pilot study in Trinidad, West Indies. For gathering prevalence data, such xenomonitoring is a suitable alternative to the use of human bait, which is ethically questionable. The resting mosquitoes were collected, either indoors or outdoors, using electrical and mouth aspirators. A 'cocoeya broom', made from a bunch of the midribs of coconut-palm leaves, was found to be useful in flushing out the mosquitoes resting in hard-to-reach areas within bedrooms. The rationale behind the strategy and the five-step methodology, of householder notification, mapping, preparation of equipment, mosquito collecting and laboratory processing, are described. Data from the pilot study indicate that this xenomonitoring protocol may be applicable worldwide, albeit with modifications to take account of variations in the vector species involved and their ecology and resting behaviour.