Chronic wasting disease prions in elk antler velvet.
Chronic wasting disease prions in elk antler velvet.
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DOI：
10.3201/eid1505.081458
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发表时间：
2009-05
影响因子：
11.8
通讯作者：
Telling GC
中科院分区：
文献类型：
--
作者：
Angers RC;Seward TS;Napier D;Green M;Hoover E;Spraker T;O'Rourke K;Balachandran A;Telling GC
宫颈朊病毒蛋白残基226可能是CWD发病机制的决定因素。慢性消耗性疾病（CWD）是鹿和麋鹿的传染性、致命性朊病毒疾病，持续在新的地区出现。为了探索朊病毒在鹿茸中高效传播的途径，我们利用cwd易感转基因小鼠和蛋白错误折叠循环扩增技术，检测了朊病毒在生长鹿茸（鹿茸茸）表皮顶端层的感染性。我们在患cwd的麋鹿的鹿茸中发现了朊病毒，这表明该组织可能在鹿群之间的疾病传播中起作用。食用鹿茸作为营养补充剂的人有暴露于朊病毒的风险。CWD朊病毒在表达麋鹿朊病毒蛋白的转基因小鼠中的孵育时间始终更快，这一事实表明，残基226是鹿和麋鹿朊病毒蛋白之间唯一的主要结构差异，可能是CWD发病的主要决定因素。
Residue 226 of cervid prion proteins may be a determinant of CWD pathogenesis. Chronic wasting disease (CWD) is a contagious, fatal prion disease of deer and elk that continues to emerge in new locations. To explore the means by which prions are transmitted with high efficiency among cervids, we examined prion infectivity in the apical skin layer covering the growing antler (antler velvet) by using CWD-susceptible transgenic mice and protein misfolding cyclic amplification. Our finding of prions in antler velvet of CWD-affected elk suggests that this tissue may play a role in disease transmission among cervids. Humans who consume antler velvet as a nutritional supplement are at risk for exposure to prions. The fact that CWD prion incubation times in transgenic mice expressing elk prion protein are consistently more rapid raises the possibility that residue 226, the sole primary structural difference between deer and elk prion protein, may be a major determinant of CWD pathogenesis.