CLONING AND SEQUENCING OF A GENE ENCODING CARMINOMYCIN 4-O-METHYLTRANSFERASE FROM STREPTOMYCES-PEUCETIUS AND ITS EXPRESSION IN ESCHERICHIA-COLI
CLONING AND SEQUENCING OF A GENE ENCODING CARMINOMYCIN 4-O-METHYLTRANSFERASE FROM STREPTOMYCES-PEUCETIUS AND ITS EXPRESSION IN ESCHERICHIA-COLI
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DOI：
10.1128/jb.175.12.3900-3904.1993
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发表时间：
1993-06-01
影响因子：
3.2
通讯作者：
HUTCHINSON, CR
中科院分区：
文献类型：
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作者：
MADDURI, K;TORTI, F;HUTCHINSON, CR
链霉菌柔红霉素生物合成基因簇的一部分的序列分析揭示了一个完整的开放阅读框架（dnrK），显示DNA和蛋白质序列同源性的几个O-甲基转移酶。在变铅青链霉菌和大肠杆菌中进行dnrK的表达以显示该基因编码洋红霉素4-O-甲基转移酶。推导的洋红霉素4-O-甲基转移酶蛋白显示其S-腺苷-L-甲硫氨酸辅因子的保守核苷酸结合位点。
Sequence analysis of a portion of the Streptomyces peucetius daunorubicin biosynthetic gene cluster revealed a complete open reading frame (dnrK) that showed DNA and protein sequence homology to several O-methyltransferases. Expression of dnrK in Streptomyces lividans and Escherichia coli was done to show that this gene codes for carminomycin 4-O-methyltransferase. The deduced carminomycin 4-O-methyltransferase protein shows a conserved nucleotide binding site for its S-adenosyl-L-methionine cofactor.