CIRCULATING FIBROCYTES DEFINE A NEW LEUKOCYTE SUBPOPULATION THAT MEDIATES TISSUE-REPAIR
CIRCULATING FIBROCYTES DEFINE A NEW LEUKOCYTE SUBPOPULATION THAT MEDIATES TISSUE-REPAIR
复制标题
DOI：
10.1007/bf03403533
复制
发表时间：
1994-11-01
影响因子：
5.7
通讯作者：
CERAMI, A
中科院分区：
文献类型：
--
作者：
BUCALA, R;SPIEGEL, LA;CERAMI, A
背景：宿主对组织损伤的反应需要多种细胞、体液和结缔组织成分的复杂相互作用。成纤维细胞通过在损伤部位内增殖并促进瘢痕形成和受损组织的长期重塑来参与该过程。组织损伤区域的成纤维细胞通常被认为是通过从周围结缔组织募集而产生的;然而这可能不是这些细胞的唯一来源。材料和方法：长期培养贴壁的人和小鼠白细胞亚群，结合各种免疫荧光和功能分析来鉴定具有成纤维细胞样特性的血源性细胞类型。我们首次描述了一群具有成纤维细胞特性的循环细胞，它们特异性地进入组织损伤部位。这种新的细胞类型，被称为“纤维细胞”，其特征在于其独特的表型（胶原蛋白（+）/波形蛋白（+）/CD 34（+）），其快速进入从血液到皮下植入的伤口腔室，并通过其存在于结缔组织scars.Conclusions：血源性纤维细胞有助于瘢痕形成，并可能在正常的伤口修复和病理性纤维化反应中发挥重要作用。
Background: The host response to tissue injury requires a complex interplay of diverse cellular, humoral, and connective tissue elements. Fibroblasts participate in this process by proliferating within injured sites and contributing to scar formation and the longterm remodeling of damaged tissue. Fibroblasts present in areas of tissue injury generally have been regarded to arise by recruitment from surrounding connective tissue; however this may not be the only source of these cells.Materials and Methods: Long-term culture of adherent, human, and murine leukocyte subpopulations was combined with a variety of immunofluorescence and functional analyses to identify a blood-borne cell type with fibroblast-like properties.Results: We describe for the first time a population of circulating cells with fibroblast properties that specifically enter sites of tissue injury. This novel cell type, termed a ''fibrocyte,'' was characterized by its distinctive phenotype (collagen(+)/vimentin(+)/CD34(+))), by its rapid entry from blood into subcutaneously implanted wound chambers, and by its presence in connective tissue scars.Conclusions: Blood-borne fibrocytes contribute to scar formation and may play an important role both in normal wound repair and in pathological fibrotic responses.