FINGERPRINTING GENOMES USING PCR WITH ARBITRARY PRIMERS
FINGERPRINTING GENOMES USING PCR WITH ARBITRARY PRIMERS
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DOI：
10.1093/nar/18.24.7213
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发表时间：
1990-12-25
影响因子：
14.9
通讯作者：
MCCLELLAND, M
中科院分区：
文献类型：
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作者：
WELSH, J;MCCLELLAND, M
使用单个随机选择的引物和聚合酶链式反应(PCR)可以产生简单和可重复性的复杂基因组指纹。不需要先前的序列信息。该方法被称为随机引物聚合酶链式反应(AP-PCR)，包括两个低严格扩增周期，然后是高严格扩增周期。我们表明，菌株可以通过比较基因组指纹的多态来区分。通过对5种金黄色葡萄球菌、11株化脓性链球菌和3个水稻品种的24个菌株的检测，证明了该方法的通用性。
Simple and reproducible fingerprints of complex genomes can be generated using single arbitrarily chosen primers and the polymerase chain reaction (PCR). No prior sequence information is required. The method, arbitrarily primed PCR (AP-PCR), involves two cycles of low stringency amplification followed by PCR at higher stringency. We show that strains can be distinguished by comparing polymorphisms in genomic fingerprints. The generality of the method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa (rice).