Stimulation of Cl permeability in colonic crypts of Lieberkuhn measured with a fluorescent indicator.
Stimulation of Cl permeability in colonic crypts of Lieberkuhn measured with a fluorescent indicator.
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用荧光指示剂测量 Lieberkuhn 结肠隐窝中 Cl 通透性的刺激。
DOI：
10.1152/ajpgi.1993.265.3.g423
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Sathiakumar,KC
中科院分区：
文献类型：
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作者：
Halm,DR;Kirk,KL;Sathiakumar,KC
从兔远端结肠分离Lieberkuhn隐窝，并使用卤素敏感染料6-甲氧基-N-[3-磺丙基]喹啉通过荧光显微镜监测细胞Cl的变化。当用前列腺素（PG）E2刺激时，兔远端结肠积极分泌Cl和K，但对肾上腺素仅分泌K。促分泌素PGE 2和肾上腺素各自产生的表观细胞氯浓度的约一半的隐窝细胞的瞬时下降。通过在浴中用葡萄糖酸盐或Br进行短暂取代来评估对Cl的渗透性。PGE 2或肾上腺素刺激分泌后，Cl流出和Br流入增加，但仅在刺激开始时表现出细胞Cl减少的细胞中。虽然葡萄糖酸盐置换期间的Cl流出与PGE 2或肾上腺素刺激相似，但肾上腺素刺激导致葡萄糖酸盐置换2分钟后的表观Cl浓度较低。总之，这些结果支持的概念，一个特定的上皮细胞类型的隐窝中的促分泌素和Cl渗透性途径不同的前列腺素E2和肾上腺素诱导的分泌状态。
Crypts of Lieberkuhn were isolated from rabbit distal colon and the halide-sensitive dye 6-methoxy-N-[3-sulfopropyl]quinolinium was used to monitor changes in cell Cl by fluorescence microscopy. Distal colon from rabbits actively secretes Cl and K when stimulated with prostaglandin (PG) E2 but secretes only K in response to epinephrine. The secretagogues PGE2 and epinephrine each produced transient decreases of the apparent cell Cl concentration in about one-half of the crypt cells. Permeability to Cl was assessed by brief substitutions with gluconate or Br in the bath. After stimulation of secretion by PGE2 or epinephrine, Cl efflux and Br influx were increased but only in the cells that exhibited the decrease in cell Cl at the onset of stimulation. Although Cl efflux during gluconate substitution was stimulated similarly with either PGE2 or epinephrine, epinephrine stimulation led to a lower apparent Cl concentration after 2 min of gluconate substitution. Together these results support the concept that a particular epithelial cell type in the crypts responds to secretagogues and that the Cl permeability pathways differ between the secretory states induced by PGE2 and epinephrine.