LIGHT-INDUCED SHIFT AND BINDING OF S-ANTIGEN IN RETINAL RODS
LIGHT-INDUCED SHIFT AND BINDING OF S-ANTIGEN IN RETINAL RODS
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DOI：
10.3109/02713688508999993
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发表时间：
1985-01-01
影响因子：
2
通讯作者：
WINKENS, HJ
中科院分区：
文献类型：
--
作者：
BROEKHUYSE, RM;TOLHUIZEN, EFJ;WINKENS, HJ
用电免疫分析法定量测定了牛和大鼠视网膜中的S抗原。感光细胞中S抗原与视紫红质的摩尔比接近1：1。免疫荧光研究表明，光诱导的S-抗原向杆外节，在那里它集中的转变。试验表明，在这些条件下，S抗原变得基本上不溶于水，但溶于去污剂。根据以前的超微结构和目前的结果，这已被解释为光诱导的S-抗原结合到杆外段膜。显然，S-抗原与视紫红质相互作用，我们推断S-抗原可能在光转导过程中起主要作用。
S-antigen has been quantitated in bovine and rat retina by electroimmunoassay. The molar ratios S-antigen to rhodopsin in photoreceptor cells were close to 1:1. Immunofluorescence studies show that light induces a shift of S-antigen towards the rod outer segments where it concentrates. Assays indicate that S-antigen becomes largely water insoluble but detergent soluble under these conditions. On the basis of previous ultrastructural and present results, this has been interpreted as a light induced binding of S-antigen to the rod outer segment membranes. Apparently, S-antigen interacts with rhodopsin and we conclude S-antigen may play a major role in the phototransduction process.