Murine echocardiography and ultrasound imaging.
Murine echocardiography and ultrasound imaging.
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DOI：
10.3791/2100
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发表时间：
2010-08-08
期刊：
影响因子：
--
通讯作者：
Blaxall, Burns
中科院分区：
文献类型：
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作者：
Pistner, Andrew;Belmonte, Stephen;Blaxall, Burns
啮齿类动物心脏病理生理模型是研究疾病机制和新的治疗方法的重要工具。超声心动图提供了一种强大的非侵入性工具，可以连续评估活体动物的心脏形态和功能。然而，由于这些动物的体积小和心率快，在小鼠身上使用这种技术带来了独特的挑战。直到最近，很少有超声系统能够对小鼠进行高质量的超声心动图检查，并且这些系统通常缺乏获得真正定量测量所需的图像分辨率和帧速率。新发布的系统，如VisualSonics Vevo2100，为研究人员提供了新的工具，以仔细和非侵入性地研究小鼠的心脏功能。该系统生成高分辨率图像，并提供与人类患者相似的分析能力。虽然彩色多普勒已经在人类中使用了30多年，但这项有价值的技术直到最近才在啮齿动物超声中成为可能。彩色多普勒超声心动图具有广泛的应用，包括能够快速评估血管和瓣膜中的血流方向性，以及快速识别瓣膜返流。应变分析是用于定量测量局部心肌功能的关键进展。该技术具有比常规技术更早地检测病理变化或病理消退的潜力。再加上三维图像重建，整个器官的体积评估是可能的，包括可视化和心脏和血管结构的评估。与鼠兼容的对比成像还可以允许体积测量和组织灌注评估。
Rodent models of cardiac pathophysiology represent a valuable research tool to investigate mechanism of disease as well as test new therapeutics. Echocardiography provides a powerful, non-invasive tool to serially assess cardiac morphometry and function in a living animal. However, using this technique on mice poses unique challenges owing to the small size and rapid heart rate of these animals. Until recently, few ultrasound systems were capable of performing quality echocardiography on mice, and those generally lacked the image resolution and frame rate necessary to obtain truly quantitative measurements. Newly released systems such as the VisualSonics Vevo2100 provide new tools for researchers to carefully and non-invasively investigate cardiac function in mice. This system generates high resolution images and provides analysis capabilities similar to those used with human patients. Although color Doppler has been available for over 30 years in humans, this valuable technology has only recently been possible in rodent ultrasound. Color Doppler has broad applications for echocardiography, including the ability to quickly assess flow directionality in vessels and through valves, and to rapidly identify valve regurgitation. Strain analysis is a critical advance that is utilized to quantitatively measure regional myocardial function. This technique has the potential to detect changes in pathology, or resolution of pathology, earlier than conventional techniques. Coupled with the addition of three-dimensional image reconstruction, volumetric assessment of whole-organs is possible, including visualization and assessment of cardiac and vascular structures. Murine-compatible contrast imaging can also allow for volumetric measurements and tissue perfusion assessment.