Extending the dynamic range of electrochemical sensors using multiple modified electrodes
Extending the dynamic range of electrochemical sensors using multiple modified electrodes
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DOI：
10.1007/s00216-006-1022-0
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发表时间：
2007-02-01
影响因子：
4.3
通讯作者：
Gooding, J. Justin
中科院分区：
文献类型：
--
作者：
Chow, Edith;Wong, Elicia L. S.;Gooding, J. Justin
多个电极，结合化学计量学策略来校准测量响应，已被用于在比单电极更宽的动态范围内测定分析物。用于检测铜的模型系统包括用自组装单层修饰的电极。电极用铜络合物（3-巯基丙酸、硫辛酸、半胱氨酸和Gly-Gly-His肽）修饰，并使用伏安分析在纳摩尔到毫摩尔的浓度范围内测定铜。我们已经证明，通过结合四个电极的校准函数，可以获得分析物浓度的更好估计（即具有较小的方差）。测量不确定度表示为独立制备的电极，这允许商业生产和工厂校准的可能性。对目标分析物使用不同亲和力的识别元素修饰的多个电极来扩展传感器动态范围的原理是一般的，可以应用于其他分析物。
Multiple electrodes, combined with a chemometric strategy to calibrate the measurement response, have been used for the determination of an analyte across a broader dynamic range than is possible with a single electrode. The model system used for the detection of copper comprised electrodes modified with a self-assembled monolayer. The electrodes were modified with the copper-complexing species (3-mercaptopropionic acid, thioctic acid, and the peptides cysteine and Gly-Gly-His) and copper was determined over concentrations ranging from nanomolar to millimolar using voltammetric analysis. We have demonstrated that by combining the calibration functions from the four electrodes a better estimate (i.e. with smaller variance) of the concentration of the analyte is obtained. Measurement uncertainty is expressed for independently prepared electrodes, which allows the possibility of commercial production and factory calibration. The principles of using multiple electrodes modified with recognition elements with different affinities for the target analyte to extend the dynamic range of sensors is a general one that could be applied to other analytes.