Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: A pilot study
Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: A pilot study
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DOI：
10.1016/j.joen.2007.11.021
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发表时间：
2008-02-01
影响因子：
4.2
通讯作者：
Huang, George T. -J.
中科院分区：
文献类型：
--
作者：
Sonoyama, Wataru;Liu, Yi;Huang, George T. -J.
间充质干细胞（MSC）已从恒牙（牙髓干细胞或DPSC）和乳牙（来自人类脱落乳牙的干细胞）的牙髓组织中分离。我们最近在人类未成熟恒牙的根尖乳头中发现了另一种类型的间充质干细胞，称为根尖乳头干细胞（SCAP）。在这里，我们进一步的特点，根尖乳头组织和干细胞特性的SCAP使用组织学，免疫组化和免疫细胞荧光分析。我们发现，根尖乳头是独特的牙髓方面，含有较少的细胞和血管成分比那些在纸浆。在器官培养中，根尖乳头中的细胞增殖比牙髓中的细胞增殖大2- 3倍。SCAP和DPSC在成骨/牙本质分化方面与骨髓间充质干细胞一样有效，而成脂潜力较弱。SCAP的免疫表型在成骨/牙本质和生长因子受体基因谱上与DPSCs相似。双染色实验表明，STRO-1共表达的牙本质标记物，如骨涎磷蛋白，骨钙素，和生长因子FGFR 1和TGF β R1在培养的SCAR此外，SCAP表达多种神经源性标记物，如巢蛋白和神经丝M刺激后的神经介质。我们得出的结论是，SCAP与DPSC相似，但却是有效牙科干细胞/祖细胞的独特来源。它们在根的发育和根尖发生的影响进行了讨论。
Mesenchymal stem cells (MSCs) have been isolated from the pulp tissue of permanent teeth (dental pulp stem cells or DPSCs) and deciduous teeth (stem cells from human exfoliated deciduous teeth). We recently discovered another type of MSCs in the apical papilla of human immature permanent teeth termed stem cells from the apical papilla (SCAP). Here, we further characterized the apical papilla tissue and stem cell properties of SCAP using histologic, immunohistochemical, and immunocytofluorescent analyses. We found that the apical papilla is distinctive to the pulp in terms of containing less cellular and vascular components than those in the pulp. Cells in the apical papilla proliferated 2- to 3-fold greater than those in the pulp in organ cultures. Both SCAP and DPSCs were as potent in osteo/dentinogenic differentiation as MSCs from bone marrows, whereas they were weaker in adipogenic potential. The immunophenotype of SCAP is similar to that of DPSCs on the osteo/dentinogenic and growth factor receptor gene profiles. Double-staining experiments showed that STRO-1 coexpressed with dentinogenic markers such as bone sialophosphoprotein, osteocalcin, and growth factors FGFR1 and TGF beta R1 in cultured SCAR Additionally, SCAP express a wide variety of neurogenic markers such as nestin and neurofilament M upon stimulation with a neurogenic medium. We conclude that SCAP are similar to DPSCs but a distinct source of potent dental stem/progenitor cells. Their implications in root development and apexogenesis are discussed.