The genetic architecture of Drosophila sensory bristle number.
The genetic architecture of Drosophila sensory bristle number.
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作者：
C. L. Dilda;T. Mackay
我们已经映射了6个重组等基因系（RIL）作图群体中的果蝇mechanosensory刚毛数量的数量性状位点（QTL），每个RIL来自从高或低，胸侧或腹部刚毛数量选择的线和等基因野生型染色体提取的等基因染色体。在三种发育温度（18度、25度和28度）下，将所有RIL作为与选择的和野生型亲本染色体杂交的雄性和雌性F（1）后代进行评价。刚毛数量QTL分别为每个染色体，性状和环境的连锁roo转座因子标记位点，使用复合区间作图。共检测到53个QTL，其中33个影响胸肋刚毛数，31个影响腹刚毛数，11个影响两个性状。大多数QTL的效应是有条件的性别（27%），温度（14%），或性别和温度（30%）。QTL间的上位性互作也很常见。虽然许多QTL定位到与候选刚毛发育位点相同的位置，但一些QTL区域不包含明显的候选基因。这些特征与维持数量性状遗传变异的进化模型密切相关，但使理解复杂性状变异的分子遗传基础的努力变得复杂。
We have mapped quantitative trait loci (QTL) for Drosophila mechanosensory bristle number in six recombinant isogenic line (RIL) mapping populations, each of which was derived from an isogenic chromosome extracted from a line selected for high or low, sternopleural or abdominal bristle number and an isogenic wild-type chromosome. All RILs were evaluated as male and female F(1) progeny of crosses to both the selected and the wild-type parental chromosomes at three developmental temperatures (18 degrees, 25 degrees, and 28 degrees ). QTL for bristle number were mapped separately for each chromosome, trait, and environment by linkage to roo transposable element marker loci, using composite interval mapping. A total of 53 QTL were detected, of which 33 affected sternopleural bristle number, 31 affected abdominal bristle number, and 11 affected both traits. The effects of most QTL were conditional on sex (27%), temperature (14%), or both sex and temperature (30%). Epistatic interactions between QTL were also common. While many QTL mapped to the same location as candidate bristle development loci, several QTL regions did not encompass obvious candidate genes. These features are germane to evolutionary models for the maintenance of genetic variation for quantitative traits, but complicate efforts to understand the molecular genetic basis of variation for complex traits.