Eicosapentaenoic acid and docosahexaenoic acid selectively attenuate U46619-induced smooth muscle cell proliferation
Eicosapentaenoic acid and docosahexaenoic acid selectively attenuate U46619-induced smooth muscle cell proliferation
复制标题
二十碳五烯酸和二十二碳六烯酸选择性减弱 U46619 诱导的平滑肌细胞增殖
作者：
R. Pakala;R. Pakala;C. Benedict
众所周知，血管平滑肌细胞（SMC）增殖是动脉粥样硬化斑块形成的关键步骤。血栓素A2（T×A2）是由聚集的血小板和受损的血管壁释放的，在动脉粥样硬化斑块的形成中可能起重要作用。许多动物研究表明，鱼油中的n−3多不饱和脂肪酸二十碳五烯酸（EPA，20∶ 5 n −3）和二十二碳六烯酸（DHA，22∶ 6 n −3）具有抗动脉粥样硬化作用。在本研究中，我们研究了EPA和DHA对I×A2诱导的SMC增殖的影响。为了确定n−3脂肪酸的功能选择性，我们还检测了花生四烯酸（AA，20∶ 4 n −6）、γ-亚麻酸（INA，18∶ 3 n −6）和油酸（OA，18∶ 1 n −9）对T×A2诱导的SMC增殖的影响。只有LPA和DHA能抑制T×A2模拟物U46619诱导的SMC增殖。当EPA和DHA以它们存在于鲱鱼油中的比例一起添加时，EPA和DHA协同作用以阻断由TXA 2模拟物诱导的SMC增殖。这些发现表明，鱼油中的n−3多不饱和脂肪酸可能通过阻断有丝分裂原刺激的SMC增殖而发挥抗动脉粥样硬化作用。
It is well known that vascular smooth muscle cell (SMC) proliferation is a key step in atheromatous plaque formation. Thromboxane A2 (T×A2), released from aggregating platelets and an injured vessel wall, may play an important role in the development of atheromatous plaque. Many animal studies have suggested that n−3 polyunsaturated fatty acids eicosapentaenoic acid (EPA, 20∶5n−3) and docosahexaenoic acid (DHA, 22∶6n−3) present in the fish oils have antiatherosclerotic effects. In the present study, we investigated the effect of EPA and DHA on I×A2-induced SMC proliferation. To determine the functional selectivity of n−3 fatty acids, we also tested the effect of arachidonic acid (AA, 20∶4n−6), γ-linolenic acid) (INA, 18∶3n−6), and oleic acid (OA, 18∶1n−9) on T×A2-induced SMC proliferation. Only LPA and DHA prevented the SMC proliferation induced by the T×A2 mimetic U46619. When EPA and DHA were added together in the ratio in which they are present in menhaden oil, EPA and DHA acted synergistically to block the SMC proliferation induced by the TXA2-mimetic. These findings suggest that the n−3 polyunsaturated fatty acids in fish oils may exert antiatherosclerotic effects by blocking the mitogenstimulated proliferation of SMC.
血清素、血栓素 A2 和特异性受体拮抗剂对血管平滑肌细胞增殖的影响。
影响因子：
37.8
作者：
Pakala,R;Willerson,JT;Benedict,CR
通讯作者：
Benedict,CR
富含二十碳五烯酸和二十二碳六烯酸的血小板磷脂可抑制血栓素 A2/前列腺素 H2 受体结合和功能。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Swann,PG;Parent,CA;Croset,M;Fonlupt,P;Lagarde,M;Venton,DL;LeBreton,GC
通讯作者：
LeBreton,GC
影响因子：
158.5
作者：
ASCHERIO, A;RIMM, EB;WILLETT, WC
通讯作者：
WILLETT, WC