Influences of sex, rhythm and generation on the obesogenic potential of erythromycin to Drosophila melanogaster.
Influences of sex, rhythm and generation on the obesogenic potential of erythromycin to Drosophila melanogaster.
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DOI：
10.1016/j.scitotenv.2021.145315
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发表时间：
2021-01
期刊：
影响因子：
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通讯作者：
Jing Zhang;Zhenyang Yu;Jiaying Shen;Laura N. Vandenberg;D. Yin
中科院分区：
文献类型：
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作者：
Jing Zhang;Zhenyang Yu;Jiaying Shen;Laura N. Vandenberg;D. Yin
抗生素因其作为新兴污染物和环境致肥胖因素的作用而受到关注，但其环境暴露和对生物体致肥胖影响之间的几个方面仍未得到充分研究。在此，黑腹果蝇连续三代（F1 至 F3）接触红霉素（ERY，0.1 μg/L）。测量体重、昼夜节律（以羽化时间表示）和脂质代谢。 ERY 增加了所有三代幼虫中脂滴的大小。它对F1和F2代中早晨异常羽化的成虫（AM成虫）的体重有一定的抑制作用，而对下午羽化的成虫（PM成虫）的抑制作用较小。相反，它刺激了 F3 成虫的体重。值得注意的是，ERY 促进雌性早晨羽化。综合 F1 至 F3 的影响，AM 雌性和雄性成虫以及 PM 雌性成虫中酰基辅酶 A 氧化酶 (ACO) 普遍升高，而 PM 雄性成虫中酰基辅酶 A 氧化酶 (ACO) 普遍降低。葡萄糖激酶 (GCK) 在 AM 成虫的两性中普遍升高，但在 PM 雄性成虫中各代均下降。尽管性别之间存在一些差异，但 IIS 通路在 AM 成人中显示出普遍的上调，但在患有节律障碍的 PM 成人中没有显示出任何共同的变化。 AMPK 通路跨代参与，没有特定的共同变化。总的来说，ERY 对糖脂代谢中关键代谢物和酶以及遗传调控的影响取决于性别、节律和暴露产生。
Antibiotics are gaining attention due to their roles as emerging pollutants and environmental obesogens, yet several aspects between their environmental exposure and obesogenic influence on organisms remain poorly explored. Here,Drosophila melanogasterwere exposed to erythromycin (ERY, 0.1 μg/L) for three consecutive generations (F1 to F3). Body weight, circadian rhythm (represented by eclosion timing) and lipid metabolism were measured. ERY increased the size of lipid droplets in larvae of all three generations. It modestly inhibited body weight in adults that abnormally eclosed in the morning (AM adults) in the F1 and F2 generations, and the inhibition was less in adults that eclosed in the afternoon (PM adults). In contrast, it stimulated body weight in F3 adults. Notably, ERY promoted morning eclosion of females. Combining the effects from F1 to F3, acyl-CoA oxidase (ACO) was commonly increased in AM female and male adults and also in PM female ones, while it was commonly decreased in PM male adults. Glucokinase (GCK) was commonly increased in both sexes of AM adults but decreased in PM male adults across generations. The IIS pathway showed a common up-regulation in the AM adults despite some differences between sexes, but it did not show any shared changes in the PM adults with dysrhythmia. The AMPK pathway was involved across generations without particular shared changes. Collectively, the effects of ERY on the key metabolites and enzymes in glucolipid metabolism and the genetic regulations depended on sex, rhythm and exposure generation.