Aberrantly Expressed Small Noncoding RNAome in Keloid Skin Tissue.
Aberrantly Expressed Small Noncoding RNAome in Keloid Skin Tissue.
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DOI：
10.3389/fgene.2022.803083
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发表时间：
2022
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
皮肤是防止受伤和感染的器官，但也很容易受损。伤口愈合是一种微妙的平衡，如果被打破，就会导致瘢痕疙瘩的形成。小的非编码RNA （nc）可以是“管家”RNA，例如核糖体RNA和转移RNA，或“调节性”RNA，例如微RNA (miRNAs或miRs)，小核核RNA （snoRNAs）和p元素诱导的睾丸（PIWI）相互作用RNA （piRNA）类型。我们使用测序和实时逆转录-定量聚合酶链反应检测了瘢痕疙瘩皮肤组织（KST）中的五种小RNA [miR， piRNA, snoRNA，小核（sn） RNA和重复相关小干扰RNA (rasiRNA)]。所有的比较都与正常皮肤组织（通过腹部成形术获得）的表达有关。在KST中，3种pirna表达上调，6种pirna表达下调。在KST中，12个snoRNAs表达上调，2个snoRNAs表达下调。两种snrna的表达在KST中下调。在KST中，18个mirna表达上调，3个mirna表达下调。在KST中，1个rasiRNA表达上调，1个rasiRNA表达下调。我们揭示了KST中小ncrna的差异表达，这可能有助于开发新的瘢痕疙瘩治疗方法。
The skin is an organ that protects against injury and infection but can be damaged easily. Wound healing is a subtle balance which, if broken, can lead to keloid formation. Small noncoding (nc) RNAs can be of “housekeeping,” for example, ribosomal RNAs and transfer RNAs, or “regulatory,” for example, microRNAs (miRNAs or miRs), small nucleolar RNAs (snoRNAs), and P-element–induced Wimpy testis (PIWI)-interacting RNA (piRNA) types. We examined five types of small ncRNAs [miR, piRNA, snoRNA, small nuclear (sn) RNA, and repeat-associated small interfering RNA (rasiRNA)] in keloid skin tissue (KST) using sequencing and real-time reverse transcription-quantitative polymerase chain reaction. All comparisons were made in relation to expression in normal skin tissue (obtained by abdominoplasty). The expression of three piRNAs was upregulated, and the expression of six piRNAs was downregulated in KST. The expression of 12 snoRNAs was upregulated, and the expression of two snoRNAs was downregulated in KST. The expression of two snRNAs was downregulated in KST. The expression of 18 miRs was upregulated, and the expression of three miRNAs was downregulated in KST. The expression of one rasiRNA was upregulated, and the expression of one rasiRNA was downregulated in KST. We revealed the differential expression of small ncRNAs in KST, which may aid the development of new treatment for keloids.
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影响因子：
56.9
作者：
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者：
Hayashizaki, Y
影响因子：
5.4
作者：
Ma X;Chen J;Xu B;Long X;Qin H;Zhao RC;Wang X
通讯作者：
Wang X
影响因子：
4.5
作者：
Askarian-Amiri, Marjan E.;Crawford, Joanna;Mattick, John S.
通讯作者：
Mattick, John S.
影响因子：
7
作者：
Dvinge, Heidi;Guenthoer, Jamie;Bradley, Robert K.
通讯作者：
Bradley, Robert K.
影响因子：
3.1
作者：
Huang, Heping;Fu, Shangfeng;Liu, Dewu
通讯作者：
Liu, Dewu