MUTATIONAL ANALYSIS OF THE TOBACCO MOSAIC-VIRUS 5'-LEADER FOR ALTERED ABILITY TO ENHANCE TRANSLATION
MUTATIONAL ANALYSIS OF THE TOBACCO MOSAIC-VIRUS 5'-LEADER FOR ALTERED ABILITY TO ENHANCE TRANSLATION
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DOI：
10.1093/nar/16.3.883
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发表时间：
1988-02-11
影响因子：
14.9
通讯作者：
WILSON, TMA
中科院分区：
文献类型：
--
作者：
GALLIE, DR;SLEAT, DE;WILSON, TMA
5‘-未翻译前导序列(OMEGA.)对烟草花叶病毒(TMV)进行了序列分析，以确定与OMEGA相关的翻译增强所必需的序列。对5个缺失突变体、1个单碱基替换突变体和1个25碱基替换突变体进行了OMEGA.S增强烟草叶肉原生质体中β-葡萄糖醛酸酶(GUS)和非洲爪哇卵母细胞中氯霉素乙酰转移酶(CAT)基因表达的能力的研究。与第二个核糖体结合所需的8个碱基亚序列的改变导致非洲爪哇卵母细胞的翻译增强丧失，但在原生质体中没有。
Mutational analysis of the 5''-untranslated leader sequence (.OMEGA.) of tobacco mosaic virus (TMV) was carried out to determine those sequences necessary for the translational enhancement associated with .OMEGA.. Five deletion mutants, a single base substitution, and a 25 base replacement mutant were tested for alterations in .OMEGA.''s ability to enhance expression of .beta.-glucuronidase (GUS) mRNA in tobacco mesophyll protoplasts and Escherichia coli or chloramphenicol acetyltransferase (CAT) mRNA in Xenopus laevis oocytes. Alteration of an eight base subsequence required for the binding of a second ribosome resulted in the loss of translational enhancement in X. laevis oocytes but not in protoplasts. Substantial increases in enhancement were observed for several of the mutants in E. coli.