Recent advances in surface plasmon resonance based techniques for bioanalysis
Recent advances in surface plasmon resonance based techniques for bioanalysis
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DOI：
10.1007/s00216-006-1052-7
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发表时间：
2007-03-01
影响因子：
4.3
通讯作者：
Cheng, Quan
中科院分区：
文献类型：
--
作者：
Phillips, K. Scott;Cheng, Quan
表面等离子体共振（SPR）是一种功能强大、用途广泛的生物分子相互作用分析（BIA）方法，近年来得到了广泛的应用。这更新的2006年SPR，SPR光谱学和SPR成像的审查探讨了尖端技术，重点是材料，方法和仪器的发展。一些最近的SPR的发展和有趣的应用生物分析提供。三个重点议题进行了更详细的讨论，以了解最近的进展。它们包括表面等离子体荧光光谱，SPR基板的纳米玻璃化，和SPR成像中的酶放大。通过这些例子，我们可以清楚地看到，基于SPR的方法的发展在不断增长，而应用也在不断多样化。主要的趋势似乎是目前在开发组合技术，使用新材料，和开发新的方法。总之，这些工作构成了一个主要的推力，最终可能使SPR表面相互作用分析和生物传感的常用工具。SPR和SPR相关的BIA研究的未来前景，在我们看来，是非常光明的。
Surface plasmon resonance (SPR) is a powerful and versatile spectroscopic method for biomolecular interaction analysis (BIA) and has been well reviewed in previous years. This updated 2006 review of SPR, SPR spectroscopy, and SPR imaging explores cutting-edge technology with a focus on material, method, and instrument development. A number of recent SPR developments and interesting applications for bioanalysis are provided. Three focus topics are discussed in more detail to exemplify recent progress. They include surface plasmon fluorescence spectroscopy, nanoscale glassification of SPR substrates, and enzymatic amplification in SPR imaging. Through these examples it is clear to us that the development of SPR-based methods continues to grow, while the applications continue to diversify. Major trends appear to be present in the development of combined techniques, use of new materials, and development of new methodologies. Together, these works constitute a major thrust that could eventually make SPR a common tool for surface interaction analysis and biosensing. The future outlook for SPR and SPR-associated BIA studies, in our opinion, is very bright.