Induction of Antifouling Diterpene Production by Streptomyces cinnabarinus PK209 in Co-Culture with Marine-Derived Alteromonas sp KNS-16
Induction of Antifouling Diterpene Production by Streptomyces cinnabarinus PK209 in Co-Culture with Marine-Derived Alteromonas sp KNS-16
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DOI：
10.1271/bbb.120221
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发表时间：
2012-10-01
影响因子：
1.6
通讯作者：
Kim, Myoung Sug
中科院分区：
文献类型：
--
作者：
Cho, Ji Young;Kim, Myoung Sug
从海洋放线菌PK 209中分离到一种活性的双萜类化合物，并通过共培养实验进行了诱导。通过核磁共振和质谱数据的解析，确定其活性成分为二萜类的Lobocytol。设计PK 209共培养物，并选择Lobocytol抗性细菌KNS-16作为共培养竞争物以诱导Lobocytol产生。将小体积的16小时龄的KNS-16培养物添加到96小时龄的PK 209培养物中引起Lobocytol生产的快速诱导。最终产量为2.7 mg/L，比从单个PK 209培养物收集的产量高10.4倍。经16 S rDNA序列测定，确定菌株PK 209和KNS-16分别为朱砂链霉菌（Streptomyces cinnabarinus）和交替单胞菌（Alteromonas sp.）。Lobocytol显示出显著的抑制活性，分别为0.18和0.43 μ g/mL，对大型藻类孔石莼和硅藻舟形藻的EC 50。其对污损细菌的MIC为61-112 μ g/mL。
An active antifouling diterpene was isolated from marine actinomycete strain PK209 and productivity was induced in a co-culture experiment. The active constituent was identified as the diterpene lobocompactol by interpretion of nuclear magnetic resonance and mass spectroscopy data. A PK209 co-culture was designed and a lobocompactol-resistant bacterium, KNS-16, was selected as co-culture competitor to induce lobocompactol production. Adding a small volume of 16-h-old KNS-16 culture to the 96-h-old PK209 culture caused rapid induction of lobocompactol production. The final yield was 2.7 mg/L, 10.4-fold higher than that collected from a single PK209 culture. The two bacteria, strains PK209 and KNS-16, were identified as Streptomyces cinnabarinus and Alteromonas sp. based on 16S rDNA sequencing. Lobocompactol showed significant antifouling activity, of 0.18 and 0.43 mu g/mL, for EC50 against the macroalga Ulva pertusa and the diatom Navicula annexa respectively. It showed activity with MIC of 61-112 mu g/mL against fouling bacteria.