Displacement chromatography on cyclodextrin-silicas. I. Separation of positional and geometrical isomers in the reversed-phase mode.
Displacement chromatography on cyclodextrin-silicas. I. Separation of positional and geometrical isomers in the reversed-phase mode.
复制标题
环糊精-二氧化硅上的置换色谱法。
DOI：
10.1016/s0021-9673(01)88971-8
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Farkas,G
中科院分区：
文献类型：
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作者：
Vigh,G;Quintero,G;Farkas,G
本文研究了几种带电和不带电溶质在β-环糊精-硅胶上的保留行为与洗脱液的甲醇浓度、离子强度和pH值的关系，以发展位置异构体和几何异构体的有效置换色谱分离。这些保留曲线用于预测导致溶质保留超过k ′ = 10的洗脱液（载体溶剂）组成。测定了几种阳离子洗涤剂在这些载体溶液中的吸附等温线，发现吸附等温线是凸的。几个位置异构体作为测试溶质的吸附等温线也被确定在这些载体溶液中。吸附等温线允许开发有效的置换色谱分离的异构体测试。在常规4.6 mm I.D.上实现高达58 mg的柱负载。分析规模的环糊精硅胶柱。
The retention behaviour of several charged and uncharged solutes on β-cyclodextrin-silica was studied as a function of the methanol cocentration, ionic strength and pH of the eluent in order to develop efficient displacement chromatographic separations for positional and geometric isomers. These retention curves were used to predict the eluent (carrier solvent) compositions that result in solute retentions in excess ofk′ = 10. The adsorption isotherms of several cationic detergents were determined in these carrier solutions and were found to be convex. The adsorption isotherms of several positional isomers used as test solutes were also determined in these carrier solutions. The adsorption isotherms permitted the development of efficient displacement chromatographic separations for the isomers tested. Columns loadings as high as 58 mg were achieved on a regular 4.6 mm I.D. analytical-scale cyclodextrin silica columns.