Molecular cloning and characterization of a PR-5 like protein gene from Brassica campestris ssp. chinensis
Molecular cloning and characterization of a PR-5 like protein gene from Brassica campestris ssp. chinensis
复制标题
DOI：
10.1007/s10535-018-0820-5
复制
发表时间：
2018-07
影响因子：
1.5
通讯作者：
C. Liu;H. Liu;Y. Wang;D. Hu;D. Xiao;C. -. Zhang;X. Hou;Y. Li
中科院分区：
文献类型：
--
作者：
C. Liu;H. Liu;Y. Wang;D. Hu;D. Xiao;C. -. Zhang;X. Hou;Y. Li
霜霉病是不结球白菜上的一种严重真菌病害。（学名：ssp.chinensisMakino）。PR-5基因在植物抗病性中起重要作用。本研究从不结球白菜中成功克隆了一个编码病程相关5-like（PR-5L）蛋白的基因，命名为BcPR-5L。BcPR-5L的cDNA序列长747 bp。该基因编码的蛋白质分子量为25.78 kDa，等电点为4.42，含248个氨基酸。多序列比对结果表明，BcPR-5L蛋白与13种不同植物中的PR-5L蛋白同源性较高，其中与芜菁的同源性最高。我们利用洋葱表皮细胞分析了BcPR-5L蛋白的亚细胞定位，发现它定位在细胞膜上。真实的时间定量PCR分析显示，H.结果表明，该基因在抗病品种中的表达量高于感病品种。综上所述，BcPR-5L基因可能在不结球白菜对H.寄生虫感染
Downy mildew caused byHyaloperonospora parasiticais a serious fungal disease in non-heading Chinese cabbage (Brassica campestrisL. ssp.chinensisMakino). Pathogenesis-related 5 (PR-5) genes play an important role in plant resistance to disease invasion. In this study, a gene encoding pathogenesis-related 5-like (PR-5L) protein, namedBcPR-5L, was successfully cloned from non-heading Chinese cabbage. The cDNA sequence ofBcPR-5Lwas 747 bp in length. It encoded a protein of molecular mass of 25.78 kDa, an isoelectric point of 4.42, and containing 248 amino acids. Multiple sequence alignment indicated that BcPR-5L protein was highly homologous to other PR-5L proteins identified in 13 different species, with the highest homology toBrassica rapa. We analyzed the subcellular localization of BcPR-5L protein by using onion epidermal cells and found that it was localized in the membrane. Real time quantitative PCR analyses revealed that the expression ofBcPR-5Lgene was significantly upregulated afterH. parasiticainfection, and the expression in the resistant cultivar was higher than that in the susceptible cultivar. In summary, our data suggest thatBcPR-5Lgene may play an important role in the resistance of non-heading Chinese cabbage toH. parasiticainfection.