RECONSTITUTION OF CELL MEMBRANE STRUCTURE IN VITRO AND ITS TRANSFORMATION INTO AN EXCITABLE SYSTEM
RECONSTITUTION OF CELL MEMBRANE STRUCTURE IN VITRO AND ITS TRANSFORMATION INTO AN EXCITABLE SYSTEM
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DOI：
10.1038/194979a0
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发表时间：
1962-01-01
期刊：
影响因子：
64.8
通讯作者：
WESCOTT, WC
中科院分区：
文献类型：
--
作者：
MUELLER, P;RUDIN, DO;WESCOTT, WC
在0.1 M盐水溶液中，通过类似于在空气肥皂膜中形成胡克-牛顿“二次黑”的方法，常规地形成了面积达10 mm.2 in的单一、稳定的双分子脂质和蛋白脂质1膜2 -4。通过在两个充满盐水的隔室之间形成这样的膜，可以测量其横向电特性，并且可以进行受控的化学研究。
THE formation of single, stable bimolecular lipid and proteolipid1membranes up to 10 mm.2in area has been accomplished routinely in 0.1Msaline solution by methods analogous to the formation of Hooke-Newton ‘secondary black’ in air soap films2–4. By forming such a membrane between two compartments filled with saline its transverse electrical properties can be measured, and controlled chemical investigations can be undertaken.