Genetic Polymorphism and Zoonotic Potential of Enterocytozoon bieneusi from Nonhuman Primates in China
Genetic Polymorphism and Zoonotic Potential of Enterocytozoon bieneusi from Nonhuman Primates in China
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中国非人灵长类比氏肠细胞虫的遗传多态性及人畜共患可能性
DOI：
10.1128/aem.03845-13
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发表时间：
2014-03-01
影响因子：
4.4
通讯作者：
Xiao, Lihua
中科院分区：
文献类型：
--
作者：
Karim, Md Robiul;Wang, Rongjun;Xiao, Lihua
毕氏肠细胞虫是一种重要的人畜共患病病原体。评价E.在非人灵长类动物（NHP）中，我们检查了E.通过PCR和核糖体内转录间隔区（ITS）序列分析，对23种NHP中的bieneusi进行了分析。共检测了来自中国五省的1,386份粪便标本，其中大肠埃希氏菌（E.在食蟹猴（67.7%）、恒河猴（8.8%）、日本猕猴（33.3%）、白头叶猴（13.6%）和金丝猴（3.5%）等5种NHP动物的158例（11.4%）标本中检测到了bieneusi（P < 0.0001）。广东、云南、广西、河南和四川的感染率分别为70.2%、21.5%、8.5%、7.5%和5.6%（P < 0.0001）。圈养动物的患病率（13.7%）显著高于散养动物（5.0%）（P < 0.0001）。共检测到16种ITS基因型，包括9种已知基因型（IV，D，Henan V，Peru 8，PigEBITS 7，EbpC，Peru 11，BEB 6和I）和7种新基因型（CM 1 ~ CM 7）。常见的基因型包括CM 1、IV和D，分别在43、31和30份标本中检出。系统发育分析表明，7个已知的基因型（但不是BEB 6和I）和4个新的基因型（CM 1，CM 2，CM 3和CM 6）属于先前描述的第1组与人畜共患病的潜力。基因型CM 5和CM 7与第2组聚在一起，而基因型CM 4不属于任何先前提出的组。结论是，居住在同一地理位置的人类和NHP共享相同的E。bieneusi基因型，表明这些动物在人畜共患传播E.谢谢
ABSTRACT Enterocytozoon bieneusi is an important zoonotic pathogen. To assess the human-infective potential of E. bieneusi in nonhuman primates (NHPs), we examined the prevalence and genotype distribution of E. bieneusi in 23 NHP species by PCR and sequence analysis of the ribosomal internal transcribed spacer (ITS). A total of 1,386 fecal specimens from NHPs from five provinces in China were examined, and E. bieneusi was detected in 158 (11.4%) specimens from five NHP species, including cynomolgus monkey (67.7%), rhesus macaque (8.8%), Japanese macaque (33.3%), white-headed langur (13.6%), and golden snub-nosed monkey (3.5%) (P < 0.0001). The infection rates were 70.2%, 21.5%, 8.5%, 7.5%, and 5.6% in Guangdong, Yunnan, Guangxi, Henan, and Sichuan Provinces, respectively (P < 0.0001). The prevalence was significantly higher in captive (13.7%) than in free-range (5.0%) animals (P < 0.0001). Altogether, 16 ITS genotypes were observed, including nine known genotypes (IV, D, Henan V, Peru8, PigEBITS7, EbpC, Peru11, BEB6, and I) and seven new genotypes (CM1 to CM7). The common genotypes included CM1, IV, and D, which were detected in 43, 31, and 30 specimens, respectively. Phylogenetic analysis revealed that seven known genotypes (but not BEB6 and I) and four new genotypes (CM1, CM2, CM3, and CM6) belonged to the previously described group 1 with zoonotic potential. Genotypes CM5 and CM7 clustered with group 2, whereas genotype CM4 did not belong to any of the previously proposed groups. It was concluded that humans and NHPs residing in the same geographical location shared the same E. bieneusi genotypes, indicating a potential role of these animals in the zoonotic transmission of E. bieneusi.