GENOMES OF MURINE LEUKEMIA VIRUSES ISOLATED FROM WILD MICE
GENOMES OF MURINE LEUKEMIA VIRUSES ISOLATED FROM WILD MICE
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DOI：
10.1128/jvi.39.3.777-791.1981
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发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
LOWY, DR
中科院分区：
文献类型：
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作者：
CHATTOPADHYAY, SK;OLIFF, AI;LOWY, DR
对从野生小鼠中分离的小鼠白血病病毒（MuLV）进行了基因组研究。对来自加州[USA]田鼠的5种嗜亲性和5种嗜异嗜性MuLV、Friend MuLV以及来自Mus musculus castaneus的1种嗜亲性和1种异嗜性MuLV，推导了8.8-内切酶（kb）未整合线性病毒DNA的详细限制性内切酶图谱。一般而言，加州MuLV在其106 kb（对应于100长的末端重复序列[LTR]、gag和pol）和101 kb（7.8-8.8 kb，对应于p15 E和p15 LTR）方面相似。对于跨越6.0-7.7 kb的区域（包括编码gp 70的序列），嗜亲性MuLV与嗜亲性MuLV共享很少的酶位点，尽管加州嗜亲性MuLV在该区域中彼此高度相关，嗜亲性MuLV也是如此。嗜亲性和加州嗜亲性MuLV DNA之间的交叉杂交研究表明，它们在6.3-7.6 kb区域不同源;加州嗜亲性病毒DNA在该区域与AKR嗜亲性MuLV交叉杂交。当加州病毒DNA与AKR嗜亲性病毒DNA比较时，在整个基因组中注意到酶位点的许多差异。野生小鼠LTR的U3区与AKR MuLV中的该区域显示部分同源性。Moloney MuLV的LTR与加州MuLV的LTR高度相关; Friend MuLV的LTR似乎是两种LTR之间的重组体。分枝栗小肌分离株与从近交系小鼠分离的嗜亲性和异嗜性MuLV最密切相关。从pBR 322中的超螺旋病毒DNA在其独特的EcoRI位点克隆了一个嗜亲性MuLV DNA。分离具有1和2个LTR的病毒DNA。经EcoRI酶切后，两种类型的DNA均具有感染性。嗜亲性和嗜亲性MuLV明显不同，主要是在编码gp 70的区域。
The genomes of murine leukemia viruses (MuLV) isolated from wild mice were studied. Detailed restriction endonuclease maps of the 8.8-kilobase (kb) unintegrated linear viral DNA were derived for 5 ecotropic and 5 amphotropic MuLV from California [USA] field mice, for Friend MuLV and for 1 ecotropic and 1 xenotropic MuLV from Mus musculus castaneus. In general, the California MuLV were similar in their leftward 6 kb (corresponding to the leftward long terminal repeat [LTR], gag and pol) and rightward 1 kb (7.8-8.8 kb, corresponding to p15E and the rightward LTR). For the region spanning 6.0-7.7 kb (which includes the sequences that encode gp70) the amphotropic MuLV shared few enzyme sites with the ecotropic MuLV although the California ecotropic MuLV were highly related to each other in this region, as were the amphotropic MuLV. Cross-hybridization studies between amphotropic and California ecotropic MuLV DNA indicated that they were not homologous in the region 6.3-7.6 kb; the California ecotropic viral DNA cross-hybridized in this region to AKR ecotropic MuLV. When the California viral DNA were comapred with AKR ecotropic viral DNA, many differences in enzyme sites were noted throughout the genome. The U3 regions of the wild mouse LTR showed partial homology to this region in AKR MuLV. The LTR of Moloney MuLV was highly related to that of the California MuLV; the LTR of Friend MuLV appeared to be a recombinant between the 2 types of LTR. The M. musculus castaneus isolates were most closely related to ecotropic and xenotropic MuLV isolated from inbred mice. One amphotropic MuLV DNA was cloned from supercoiled viral DNA at its unique EcoRI site in pBR322. Viral DNA with 1 and 2 LTR were isolated. After digestion with EcoRI, DNA of both types were infectious. Ecotropic and amphotropic MuLV apparently differ primarily in the region which encodes gp70.