PROJECTIONS OF ENTOPEDUNCULAR NUCLEUS AND GLOBUS PALLIDUS IN RAT AS DEMONSTRATED BY AUTORADIOGRAPHY AND HORSERADISH-PEROXIDASE HISTOCHEMISTRY
PROJECTIONS OF ENTOPEDUNCULAR NUCLEUS AND GLOBUS PALLIDUS IN RAT AS DEMONSTRATED BY AUTORADIOGRAPHY AND HORSERADISH-PEROXIDASE HISTOCHEMISTRY
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DOI：
10.1002/cne.901770108
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发表时间：
1978-01-01
影响因子：
2.5
通讯作者：
FIBIGER, HC
中科院分区：
文献类型：
--
作者：
CARTER, DA;FIBIGER, HC
用放射自显影和辣根过氧化物酶组织化学技术研究了大鼠苍白球（GP）和脚内核（EP）的传出投射。立体定位注射后的3 H-亮氨酸到EP，运输标签中观察到的丘脑腹内侧核（VM）的外侧部分，更横向，在腹外侧的腹侧-腹前丘脑复合体（VALv）的腹外侧方面。放射自显影颗粒的选择性积累也被发现在束旁核（Pf）和外侧缰核。HRP注射到丘脑证实了脚内核投射到这些核团。在将3 H-亮氨酸注射到EP和黑质的网状核中后，丘脑中颗粒分布的比较（Clavier等人，3H-3亮氨酸进入GP导致放射自显影颗粒主要积聚在丘脑底核的内侧部分和黑质。在背侧丘脑中也观察到光和弥散标记。然而，GP注射的一致特征是，它们似乎导致同位素扩散到丘脑的网状核，并且鉴于该核的广泛丘脑投射，背侧丘脑中的标记不能肯定地归因于苍白球传出。整个丘脑注射HRP未能在GP中产生反应性细胞。因此，这些实验没有为最近提出的大鼠苍白球向丘脑有广泛投射的观点提供有力的支持。
The efferent projections of the globus pallidus (GP) and entopeduncular nucleus (EP) in the rat were studied using autoradiographic and horseradish peroxidase (HRP) histochemical techniques. Following stereotaxic injections of 3H-leucine into EP, transported label was observed in the lateral part of the ventromedial nucleus (VM) of the thalamus, and more laterally, in the ventrolateral aspect of the ventral lateral-ventral anterior thalamic complex (VALv). Selective accumulation of autoradiographic grains was also found in the parafascicular nucleus (Pf) and in the lateral habenular nucleus. HRP injections into the thalamus confirmed the entopeduncular projections to these nuclei. Comparison of grain distribution in the thalamus after 3H-leucine injections into EP and the zona reticulata of the substantia nigra (Clavier et al.,'76) indicates that there is considerable overlap between nigral and EP projections to both VM and Pf. The nigrothalamic and entopedunculothalamic projections to VM and VALv are discussed with reference to the possibility that in the rat these nuclei are homologous to specific nuclei within the ventral lateral-ventral anterior complex in primates.Injections of 3H-leucine into GP resulted in major accumulations of autoradiographic grains in the medial part of the subthalamic nucleus and the substantia nigra. Light and diffuse labelling was also observed in the dorsal thalamus. However, a consistent feature of the GP injections was that they appeared to result in diffusion of isotope to the reticular nucleus of the thalamus, and in view of the widespread thalamic projections of this nucleus, the label in dorsal thalamus could not be positively attributed to pallidal efferents. HRP injections throughout the thalamus failed to produce reactive cells in the GP. These experiments provided no firm support therefore for a recent suggestion that there are extensive projections from the globus pallidus to the thalamus in rat.