Biomolecular-motor-based autonomous delivery of lipid vesicles as nano- or microscale reactors on a chip
Biomolecular-motor-based autonomous delivery of lipid vesicles as nano- or microscale reactors on a chip
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DOI：
10.1039/c004615a
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Sutoh, Kazuo
中科院分区：
文献类型：
--
作者：
Hiyama, Satoshi;Moritani, Yuki;Sutoh, Kazuo
我们的目标是创建一个自主的芯片系统，使用生物系统的机械，作为纳米或微尺度反应器，执行脂质囊泡（脂质体）的靶向递送。反应器脂质体是实现生物分子马达生化分析芯片的理想模型载体；然而，目前还没有能够以自主方式靶向递送货物脂质体的系统。通过利用基于生物分子运动的运动性和DNA杂交，我们证明了单链DNA （ssDNA）标记的微管（MTs）在运动蛋白涂覆的表面上滑行，充当货物转运体，并且ssDNA标记的货物脂质体被装载/卸载到滑行的MTs上，而不会在装载库/ DNA碱基序列指定的微图案卸载位点上破裂。我们的研究结果有助于开发压力驱动或基于电动流动的微流体装置的替代策略。
We aimed to create an autonomous on-chip system that performs targeted delivery of lipid vesicles (liposomes) as nano-or microscale reactors using machinery from biological systems. Reactor-liposomes would be ideal model cargoes to realize biomolecular-motor-based biochemical analysis chips; however, there are no existing systems that enable targeted delivery of cargo-liposomes in an autonomous manner. By exploiting biomolecular-motor-based motility and DNA hybridization, we demonstrate that single-stranded DNA (ssDNA)-labeled microtubules (MTs), gliding on kinesin-coated surfaces, acted as cargo transporters and that ssDNA-labeled cargo-liposomes were loaded/unloaded onto/from gliding MTs without bursting at loading reservoirs/micropatterned unloading sites specified by DNA base sequences. Our results contribute to the development of an alternative strategy to pressure-driven or electrokinetic flow-based microfluidic devices.