The First Specific Detection of a Highly Pathogenic Avian Influenza Virus (H5N1) in Ivory Coast
The First Specific Detection of a Highly Pathogenic Avian Influenza Virus (H5N1) in Ivory Coast
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DOI：
10.1111/j.1863-2378.2008.01167.x
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发表时间：
2009-02-01
影响因子：
2.4
通讯作者：
Cattoli, G.
中科院分区：
文献类型：
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作者：
Couacy-Hymann, E.;Danho, T.;Cattoli, G.
象牙海岸动物疾病中心实验室的病毒学实验室在2006年2月至12月期间收到了野生和家养鸟类的样本。采用RT-PCR技术检测禽流感病毒和高致病性禽流感亚型病毒。在2125个样本中，16个样本为A型阳性，其中12个样本后来被证实为H5 N1。将16份A型阳性样品中的15份接种于11日龄鸡胚绒毛尿囊腔进行病毒分离。8株病毒血凝滴度为1/64 ~ 1/512。4/16份M-RT-PCR阳性标本（H5 N1阴性）为H7亚型阴性。该实验室监测科特迪瓦H5 N1病毒的诊断效率得到了证明。H5 N1阳性病例来自雀鹰（Accipter nisus）、活市售家禽和散养家禽，死亡率分别约为20%（2/10）和96.7%（29/30）。目前，对集约化家禽养殖场的调查证明H5 N1病毒呈阴性。这次没有人类病例报告。
The Virology Laboratory of the Central Laboratory of Animal Diseases in Ivory Coast at Bingerville received samples of wild and domestic avian species between February and December 2006. An RT-PCR technique was used to test for avian influenza (AI) and highly pathogenic AI subtype viruses. Among 2125 samples, 16 were type A positive; of which, 12 were later confirmed to be H5N1. Fifteen of these 16 type A positive samples were inoculated into the chorioallantoic cavity of 11-day-old embryonated hens' eggs for virus isolation. Eight produced virus with hemagglutination titres from 1/64 to 1/512. The 4/16 M-RT-PCR positive samples, which were H5N1 negative, were shown to be H7 subtype negative. The diagnostic efficiency of the laboratory for the surveillance of H5N1 in Ivory Coast was demonstrated. The positive cases of H5N1 were from a sparrowhawk (Accipter nisus); live market poultry and in free-range poultry, where the mortality rate was approximately 20% (2/10) and 96.7% (29/30) respectively. Currently, investigations into intensive poultry farms have proved negative for H5N1. No human cases have been reported this time.