Stem-cell-capturing collagen scaffold promotes cardiac tissue regeneration.
Stem-cell-capturing collagen scaffold promotes cardiac tissue regeneration.
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DOI：
10.1016/j.biomaterials.2010.12.026
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发表时间：
2011-04
期刊：
影响因子：
14
通讯作者：
Chunying Shi;Qingguo Li;Yannan Zhao;Wei Chen;Bing Chen;Zhifeng Xiao;Hang Lin;Ling Nie;Dongjin Wang;Jianwu Dai
中科院分区：
文献类型：
--
作者：
Chunying Shi;Qingguo Li;Yannan Zhao;Wei Chen;Bing Chen;Zhifeng Xiao;Hang Lin;Ling Nie;Dongjin Wang;Jianwu Dai
基于干细胞的治疗正在成熟。除了干细胞移植之外，使用天然自体干细胞进行组织再生也是一个目标。然而，自体干细胞在靶向部位的募集并不充分，这限制了自体干细胞的临床应用。生物材料已越来越多地用于组织修复。它们不仅作为细胞增殖、分化的支架，还为三维重建提供指导。在这项研究中，我们试图通过与干细胞特异性抗体结合的干细胞捕获胶原支架在伤口部位富集自体干细胞。Sca-1是造血干细胞、心肌干细胞和骨骼肌干细胞的共同表面标志物。由于抗体和抗原的相互作用，Sca-1阳性细胞可以在体外和体内富集到功能性胶原支架上。当将功能性胶原支架作为补片移植到C57/BL 6小鼠中以修复外科心脏缺损时，已经观察到心肌细胞的再生。因此，共价结合干细胞特异性抗体的胶原支架可能是促进组织再生的有效途径。
Stem cell based therapy is coming of age. Besides stem cell transplantation, it has been a goal to use native autologous stem cells for tissue regeneration. However, the recruitment of native autologous stem cells at the targeting site has not been sufficient which limits the clinical application of autologous stem cells. Biomaterials have been increasingly used in tissue repair. They not only serve as scaffolds for cell proliferation, differentiation, and also provide guidance for 3-D reestablishment. In this study, we have attempted to enrich autologous stem cells at the wound site through a stem-cell-capturing collagen scaffold by conjugating with a stem cell specific antibody. Sca-1 is a common surface marker of hematopoietic, cardiac and skeletal muscle stem cells. Due to the interaction of antibody and antigen, Sca-1 positive cells could be enriched to the functional collagen scaffold both in vitro and in vivo. When the functional collagen scaffold is transplanted into C57/BL6 mouse as a patch to repair a surgical heart defect, the regeneration of cardiomyocytes has been observed. Thus, the collagen scaffolds covalently conjugated with stem cell specific antibody could be an effective approach to promote tissue regeneration.