ULTRASTRUCTURE OF KIDNEY PRESERVATION: VARYING THE AMOUNT OF AN EFFECTIVE OSMOTIC AGENT IN ISOTONIC AND HYPERTONIC PRESERVATION SOLUTIONS
ULTRASTRUCTURE OF KIDNEY PRESERVATION: VARYING THE AMOUNT OF AN EFFECTIVE OSMOTIC AGENT IN ISOTONIC AND HYPERTONIC PRESERVATION SOLUTIONS
复制标题
肾脏保存的超微结构：改变等渗和高渗保存溶液中有效渗透剂的量
作者：
A. K. Coffey;P. Andrews
目前，在肾脏冷藏期间防止细胞肿胀所需的有效（即不需要的）渗透剂的水平尚不清楚。在本研究中，我们使用光镜和透射电镜来评估在24和48小时的冷藏中保护大鼠肾实质免受损伤所需的渗透剂蔗糖的量。我们的观察表明，当蔗糖对磷酸盐缓冲溶液的总os-摩尔浓度的贡献达到140 mmol或更多时，大多数细胞肿胀和相关的超微结构损伤可以在48小时的冷藏中得到预防。我们还发现，当肾脏储存在等渗（300 mOsmol）溶液中与高渗（400 mOsmol）溶液中含有相同量的蔗糖时，肾脏超微结构保存的质量几乎没有差异。含有140或200 mOsmol蔗糖的溶液的整体保存质量明显优于我们之前观察到的临床流行的肾脏保存溶液，如Collins， Euro-Collins和Sacks。
Currently the level of effective (i.e., impermeant) osmotic agent necessary to prevent cell swelling during the cold storage of kidneys is not known. In the present investigation, we used light microscopy and transmission electron microscopy to evaluate the amount of the osmotic agent sucrose which is needed to protect the rat kidney parenchyma from damage over 24 and 48 hr of cold storage. Our observations indicate that when sucrose contributes 140 mOsmol or more to the total os-molality of phosphate-buffered solutions, most cell swelling and associated ultrastructural damage can be prevented over 48 hr of cold storage. We also found little difference between the quality of kidney ultrastructural preservation which results when kidneys are stored in isotonic (300 mOsmol) versus hypertonic (400 mOsmol) solutions that contain the same amount of sucrose. The overall quality of preservation seen with solutions which contain 140 or 200 mOsmol of sucrose is dramatically better than that which we previously observed with such clinically popular kidney preservation solutions as Collins, Euro-Collins, and Sacks.