COMPARATIVE HUMAN CELLULAR RADIOSENSITIVITY .1. THE EFFECT OF SV40 TRANSFORMATION AND IMMORTALIZATION ON THE GAMMA-IRRADIATION SURVIVAL OF SKIN DERIVED FIBROBLASTS FROM NORMAL INDIVIDUALS AND FROM ATAXIA-TELANGIECTASIA PATIENTS AND HETEROZYGOTES
COMPARATIVE HUMAN CELLULAR RADIOSENSITIVITY .1. THE EFFECT OF SV40 TRANSFORMATION AND IMMORTALIZATION ON THE GAMMA-IRRADIATION SURVIVAL OF SKIN DERIVED FIBROBLASTS FROM NORMAL INDIVIDUALS AND FROM ATAXIA-TELANGIECTASIA PATIENTS AND HETEROZYGOTES
复制标题
DOI：
10.1080/09553008814552321
复制
发表时间：
1988-12-01
影响因子：
2.6
通讯作者：
MAYNE, LV
中科院分区：
文献类型：
--
作者：
ARLETT, CF;GREEN, MHL;MAYNE, LV
我们比较了 22 个原代人成纤维细胞株、9 个 SV40 永生化人成纤维细胞系和 7 个 SV40 转化的危机前人成纤维细胞培养物中 60Co 伽马射线照射后的细胞杀伤情况。我们检查了来自正常个体、共济失调毛细血管扩张 (A-T) 患者和 A-T 杂合子的材料。我们已经证实 A-T 衍生细胞对伽马辐射具有更高的敏感性。 A-T 细胞和正常细胞之间的区别在 SV40 病毒永生化的细胞中得以保留，但永生化细胞比相应的原代成纤维细胞更能抵抗伽马辐射。由表达 SV40 T 抗原的质粒（pSV3 gpt 和 pSV3neo）转化的细胞，无论是危机前还是危机后，都显示出这种增加的抗性，表明是 SV40 T 抗原的表达，而不是永生化本身造成了这种变化。我们使用从直线拟合获得的 Do 和从多目标曲线估计的 .hivin.D 作为参数来比较放射敏感性。我们建议两者各有优点； Do 可能更具可重复性，但在比较有肩数据和无肩数据时，.hivin.D 更现实。
We have compared cell killing following 60Co gamma irradiation in 22 primary human fibroblast strains, nine SV40-immortalized human fibroblast lines and seven SV40-transformed pre-crisis human fibroblast cultures. We have examined material from normal individuals, from ataxia-telangiectasia (A-T) patients and from A-T heterozygotes. We have confirmed the greater sensitivity of A-T derived cells to gamma radiation. The distinction between A-T and normal cells is maintained in cells immortalized by SV40 virus but the immortal cells are more gamma radiation resistant than the corresponding primary fibroblasts. Cells transformed by plasmids (pSV3 gpt and pSV3neo) expressing SV40 T-antigen, both pre- and post-crisis, show this increased resistance, indicating that it is expression of SV40 T-antigen, rather than immortalization per se which is responsible for the change. We use Do, obtained from a straight line fit, and .hivin.D, estimated from a multitarget curve, as parameters to compare radiosensitivity. We suggest that both have their advantages; Do is perhaps more reproducible, but .hivin.D is more realistic when comparing shouldered and non-shouldered data.