Growth hormone regulates the abundance of insulin-like growth factor I RNA in adult rat liver.
Growth hormone regulates the abundance of insulin-like growth factor I RNA in adult rat liver.
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DOI：
10.1016/s0021-9258(18)67484-8
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发表时间：
1986-08
期刊：
影响因子：
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通讯作者：
Charles T. Roberts;A. L. Brown;D. Graham;Steven Seelig;Susan A. Berry;K. Gabbay;M. Rechler
中科院分区：
文献类型：
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作者：
Charles T. Roberts;A. L. Brown;D. Graham;Steven Seelig;Susan A. Berry;K. Gabbay;M. Rechler
胰岛素样生长因子I（IGF-I）是存在于人和大鼠血浆中的促有丝分裂多肽，被认为介导垂体生长激素对软骨的作用以促进骨骼伸长。在大鼠中，IGF-I的血浆水平显示发育和激素调节：水平在出生时较低，随年龄增长而增加，在生长激素缺乏的成年动物中降低。本研究表明，这些变化在血浆IGF-I反映了丰富的IGF-I RNA在大鼠肝脏。人IGF-I cDNA探针与成年大鼠肝脏中大小为8.6、4.6、3.2、2.1和1.0-1.4个酶的多种RNA种类杂交。这些RNA种类在新生大鼠（2天和12天大）肝脏中减少了80%以上，在通过垂体切除术导致生长激素缺乏的成年大鼠肝脏中减少了90%以上。用生长激素治疗垂体切除大鼠增加了所有种类IGF-I RNA的丰度。这些结果表明，生长激素调节其生理介质的表达，通过改变合成，稳定性，或两者的IGF-I RNA在大鼠肝脏。
Insulin-like growth factor I (IGF-I) is a mitogenic polypeptide present in the plasma of man and rat that is thought to mediate the actions of pituitary growth hormone on cartilage to promote skeletal elongation. In the rat, plasma levels of IGF-I show both developmental and hormonal regulation: levels are low at birth, increase with age, and are decreased in growth hormone-deficient adult animals. The present study demonstrates that these changes in plasma IGF-I reflect the abundance of IGF-I RNA in rat liver. A human IGF-I cDNA probe hybridized to multiple RNA species in adult rat liver with sizes 8.6, 4.6, 3.2, 2.1, and 1.0-1.4 kilobases. These RNA species were decreased by greater than 80% in neonatal (2- and 12-day-old) rat liver and by greater than 90% in liver from adult rats made growth hormone-deficient by hypophysectomy. Treatment of hypophysectomized rats with growth hormone increased the abundance of all species of IGF-I RNA. These results suggest that growth hormone regulates the expression of its physiological mediator by altering the synthesis, stability, or both of IGF-I RNA in rat liver.