No evidence for a circadian rhythm of protein synthesis in the rat suprachiasmatic nuclei.
No evidence for a circadian rhythm of protein synthesis in the rat suprachiasmatic nuclei.
复制标题
没有证据表明大鼠视交叉上核中蛋白质合成存在昼夜节律。
DOI：
10.1016/0006-8993(89)90155-8
复制
发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Smith,CB
中科院分区：
文献类型：
--
作者：
Scammell,TE;Schwartz,WJ;Smith,CB
哺乳动物的视交叉上核含有内源性昼夜节律起搏器。使用测量1-[1-14C]亮氨酸掺入蛋白质的定量放射自显影示踪剂方法来确定细胞核中蛋白质合成的总体速率是否以昼夜节律方式变化。与先前在两个采样点的细胞核记录的电活动和能量代谢的稳健昼夜节律不同，蛋白质合成保持恒定。
The mammalian suprachiasmatic nuclei contain an endogenous circadian pacemaker. A quantitative autoradiographic tracer method that measuresl-[1-14C]leucine incorporation into protein was used to determine whether the overall rate of protein synthesis in the nuclei varies in a circadian fashion. Unlike the robust circadian rhythms of electrical activity and energy metabolism previously recorded from the nuclei at the two points sampled, protein synthesis remained constant.