Risk assessment of the transfer of imazethapyr herbicide tolerance from Clearfield rice to red rice (Oryza sativa)
Risk assessment of the transfer of imazethapyr herbicide tolerance from Clearfield rice to red rice (Oryza sativa)
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DOI：
10.1007/s10681-006-9180-x
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发表时间：
2006-10
期刊：
影响因子：
1.9
通讯作者：
Weiqiang Zhang;S. Linscombe;E. Webster;Siyuan Tan;J. Oard
中科院分区：
文献类型：
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作者：
Weiqiang Zhang;S. Linscombe;E. Webster;Siyuan Tan;J. Oard
克利尔菲尔德水稻与杂草红稻的杂交将直接影响咪草烟技术用于水稻杂草控制的管理和长期策略。本研究的目的是确定克利尔菲尔德水稻和红稻异交的比率和农艺后果。红稻群体在株高、穗长、单株分蘖数、单株粒数、结实率和粒重等性状上表现出广泛的变异。从所有克利尔菲尔德水稻品种（“CL 121”、“CL 141”、“CL 161”和“CLXL 8”）中检测到与红米的异交，并通过表型和DNA标记分析证实。2002年红米的异交频率为0.17%，异交频率在0%~ 0.46%之间。2002年红米样品对咪草烟的耐受性与乙酰羟酸合成酶（AHAS）活性水平相对应。大多数（94%）的后代从2002年的样品分离3抗：1易受耐咪唑乙烟酸，表明一个单一的显性基因从克利尔菲尔德水稻与杂交材料的耐受性。其余的样品没有分离的耐受性，表明自发突变的耐受性存在于此材料之前或之后与克利尔菲尔德水稻杂交。2003年红米的异交频率增加了4倍，为0.68%，其中单个地点的异交频率最高，为3.2%。这项研究的结果表明，异交之间的克利尔菲尔德和红米将迅速发生的速度，保证早季田间侦察和作物轮作计划，以延长有用的克利尔菲尔德技术。
Hybridization between Clearfield rice and weedy red rice would have a direct impact on management and long-term strategies of imazethapyr technology for rice weed control. The objective of this research was to determine rates and agronomic consequences for outcrossing between Clearfield rice and red rice. Red rice populations showed extensive variation for plant height, panicle length, tillers/plant, seeds/plant, seed set and grain weight. Outcrossing was detected from all Clearfield rice cultivars (‘CL121’, ‘CL141’, ‘CL161’, and ‘CLXL8’) to red rice and was confirmed by phenotypic and DNA marker analyses. An overall outcrossing frequency of 0.17% was observed in 2002 red rice samples with a range from 0% to 0.46%. Tolerance of 2002 red rice samples to imazethapyr corresponded to levels of acetohydroxyacid synthase (AHAS) activity. A majority (94%) of the progeny from the 2002 samples segregated 3 resistant:1 susceptible for tolerance to imazethapyr, indicating that a single dominant gene from Clearfield rice was associated with tolerance in the hybrid material. The remaining samples did not segregate for tolerance, suggesting that spontaneous mutations for tolerance were present in this material before or after crossing with Clearfield rice. A four-fold increase in outcrossing frequency of 0.68% was observed in 2003 red rice samples with the highest outcrossing frequency for a single location at 3.2%. Results from this study indicate that outcrossing between Clearfield and red rice will occur rapidly at rates that warrant early-season field scouting and a crop rotation scheme to prolong usefulness of the Clearfield technology.