Gene expression comparison of resistant and susceptible Atlantic salmon fry challenged with Infectious Pancreatic Necrosis virus reveals a marked contrast in immune response.
Gene expression comparison of resistant and susceptible Atlantic salmon fry challenged with Infectious Pancreatic Necrosis virus reveals a marked contrast in immune response.
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DOI：
10.1186/s12864-016-2600-y
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发表时间：
2016-04-11
期刊：
影响因子：
4.4
通讯作者：
Houston RD
中科院分区：
文献类型：
--
作者：
Robledo D;Taggart JB;Ireland JH;McAndrew BJ;Starkey WG;Haley CS;Hamilton A;Guy DR;Mota-Velasco JC;Gheyas AA;Tinch AE;Verner-Jeffreys DW;Paley RK;Rimmer GS;Tew IJ;Bishop SC;Bron JE;Houston RD
传染性胰腺坏死症（Infectious Pancreatic Necrosis，IPN）是一种由双RNA病毒引起的鲑科鱼类的高度传染性疾病。先前已经描述了大西洋鲑鱼（萨尔莫salar L.）的大的宿主抗性遗传组分，这导致了一些家庭的高死亡率和其他家庭的零死亡率。然而，这种耐药性的分子和免疫学基础尚不完全清楚。这份手稿描述了一个全球性的比较耐药和易感大西洋鲑鱼鱼苗的基因表达谱与IPN病毒的挑战。用病毒攻击来自两个IPNV抗性和两个IPNV敏感的全同胞家庭的鲑鱼鱼苗，并在攻击后1天、7天和20天取样。在所有时间点，在抗性和易感鱼中均观察到显著的病毒滴度，尽管易感鱼中的病毒滴度通常较高。基因表达谱结合基因本体和途径分析表明，虽然在抗性和易感鱼中观察到明确的免疫应答，但两种表型之间存在显着差异。易感鱼表现出与细胞因子活性和炎症反应相关的基因的显著上调，这显然未能保护免受病毒的侵害。相反，抗性鱼表现出不太明显的免疫应答，包括与M2巨噬细胞系统相关的基因的上调。虽然只有易感表型显示出可观的死亡率水平，但抗性和易感鱼都可以感染IPNV。易感鱼类的特点是免疫反应大得多，但无效，主要与细胞因子和炎症系统有关。耐药鱼表现出更温和的，推定的巨噬细胞介导的炎症反应，这可能有助于他们的生存。本文的在线版本（doi：10.1186/s12864-016-2600-y）包含补充材料，可供授权用户使用。
Infectious Pancreatic Necrosis (IPN) is a highly contagious birnavirus disease of farmed salmonid fish, which often causes high levels of morbidity and mortality. A large host genetic component to resistance has been previously described for Atlantic salmon (Salmo salar L.), which mediates high mortality rates in some families and zero mortality in others. However, the molecular and immunological basis for this resistance is not yet fully known. This manuscript describes a global comparison of the gene expression profiles of resistant and susceptible Atlantic salmon fry following challenge with the IPN virus. Salmon fry from two IPNV-resistant and two IPNV-susceptible full sibling families were challenged with the virus and sampled at 1 day, 7 days and 20 days post-challenge. Significant viral titre was observed in both resistant and susceptible fish at all timepoints, although generally at higher levels in susceptible fish. Gene expression profiles combined with gene ontology and pathway analyses demonstrated that while a clear immune response was observed in both resistant and susceptible fish, there were striking differences between the two phenotypes. The susceptible fish showed marked up-regulation of genes related to cytokine activity and inflammatory response that evidently failed to protect against the virus. In contrast, the resistant fish demonstrated a less pronounced immune response including up-regulation of genes relating to the M2 macrophage system. While only the susceptible phenotype shows appreciable mortality levels, both resistant and susceptible fish can become infected with IPNV. Susceptible fish are characterized by a much larger, yet ineffective, immune response, largely related to cytokine and inflammatory systems. Resistant fish demonstrate a more moderate, putative macrophage-mediated inflammatory response, which may contribute to their survival. The online version of this article (doi:10.1186/s12864-016-2600-y) contains supplementary material, which is available to authorized users.