Pancreas-specific aquaporin 12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis
Pancreas-specific aquaporin 12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis
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DOI：
10.1152/ajpcell.00117.2009
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发表时间：
2009-12-01
影响因子：
5.5
通讯作者：
Rai, Tatemitsu
中科院分区：
文献类型：
--
作者：
Ohta, Eriko;Itoh, Tomohiro;Rai, Tatemitsu
Ohta E, Itoh T, Nemoto T, Kumagai J, Ko SB, Ishibashi K, Ohno M, Uchida K, Ohta A, Sohara E, Uchida S, Sasaki S, Rai T.胰腺特异性水通道蛋白12缺失小鼠对小蛋白诱导的急性胰腺炎的易感性增加。[J] .中国生物医学工程学报，2009,31(2):368- 368。2009年9月2日首次发表；doi: 10.1152 / ajpcell.00117.2009。-水通道蛋白12 （AQP12）是最近发现的哺乳动物AQP家族成员，在胰腺腺泡细胞中特异性表达。体外表达研究表明AQP12定位于细胞内位点。为了确定AQP12在胰腺中的生理作用，我们产生了该基因的敲除小鼠（AQP12- ko）。野生型（WT）和AQP12-KO小鼠在正常条件下在生长、血液化学、胰液含量或组织学方面没有明显差异。然而，当我们通过给药胆囊收缩素-8 （CCK-8）类似物诱导胰腺炎时，AQP12-KO小鼠对该器官的病理损伤比WT小鼠更严重。此外，当我们使用双光子激发成像方法分析胰腺腺泡分泌时，结果显示高CCK-8剂量（100 nM）下AQP12-KO小鼠的腺泡中有更大的分泌囊泡（液泡）。根据这些结果，我们得出结论，AQP12可能在快速和强烈刺激后控制胰液正常分泌的机制中起作用。
Ohta E, Itoh T, Nemoto T, Kumagai J, Ko SB, Ishibashi K, Ohno M, Uchida K, Ohta A, Sohara E, Uchida S, Sasaki S, Rai T. Pancreas-specific aquaporin 12 null mice showed increased susceptibility to caerulein-induced acute pancreatitis. Am J Physiol Cell Physiol 297: C1368-C1378, 2009. First published September 2, 2009; doi:10.1152/ajpcell.00117.2009.-Aquaporin 12 (AQP12) is the most recently identified member of the mammalian AQP family and is specifically expressed in pancreatic acinar cells. In vitro expression studies have revealed that AQP12 is localized at intracellular sites. To determine the physiological roles of AQP12 in the pancreas, we generated knockout mice for this gene (AQP12-KO). No obvious differences were observed under normal conditions between wild-type (WT) and AQP12-KO mice in terms of growth, blood chemistry, pancreatic fluid content, or histology. However, when we induced pancreatitis through the administration of a cholecystokinin-8 (CCK-8) analog, the AQP12-KO mice showed more severe pathological damage to this organ than WT mice. Furthermore, when we analyzed exocytosis in the pancreatic acini using a two-photon excitation imaging method, the results revealed larger exocytotic vesicles (vacuoles) in the acini of AQP12-KO mice at a high CCK-8 dose (100 nM). From these results, we conclude that AQP12 may function in the mechanisms that control the proper secretion of pancreatic fluid following rapid and intense stimulation.