Changes in endocrine profile and reproductive organs during puberty in the male marmoset monkey (Callithrix jacchus)
Changes in endocrine profile and reproductive organs during puberty in the male marmoset monkey (Callithrix jacchus)
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DOI：
10.1530/rep.1.01186
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发表时间：
2006-08-01
期刊：
影响因子：
3.8
通讯作者：
Simoni, Manuela
中科院分区：
文献类型：
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作者：
Chandolia, Ramesh K.;Luetjens, Craig Marc;Simoni, Manuela
关于雄性绒猴（人类生殖模型）青春期成熟的数据很少，而且相互矛盾。我们收集了表征青春期的新参数数据。按周（wk）将25只绒猴分为5个年龄组：21（青春期前）、43（青春期开始）、52（完全青春期）、70（成熟）和116（完全成年）。测定血清和睾丸内睾酮和垂体生物活性绒毛膜促性腺激素（bioCG）。通过超声检查、组织学和流式细胞术评估睾丸发育。三个连续的血液样本显示睾丸激素浓度的极端波动，表明不稳定的分泌。观察到血清睾酮和垂体bioCG浓度的变化。睾丸内雄激素（ITAs）在所有年龄组内表现出较高的波动，并在21周的一些动物中较高。出乎意料的是，垂体bioCG和血清睾酮或ITAs之间没有相关性，但这些参数与睾丸重量和体积显着相关。这些观察结果与睾丸生长对bioCG的依赖性一致。不幸的是，在本研究中无法测量低血清bioCG水平。43周龄时，动物进入青春期。在52周龄时，动物达到最大体重和附睾重量，精子发生质量正常，但睾丸继续生长，在70周龄时达到所有参数的最大值，在116周龄时没有进一步的重大变化。结论是：（1）性腺激活是明显的，在第21周，（2）雄性绒猴达到青春期阈值约43周龄，达到定性参数在52周龄，进一步成熟到性成熟在70周龄，（3）血清睾酮和ITAs是高度可变的，没有任何可识别的相关性与垂体bioCG。
Data on pubertal maturation in male marmoset, a model for human reproduction, are scant and conflicting. We collected data on novel parameters to characterize puberty. Twenty-five marmoset monkeys were assigned to five age groups by weeks (wk): 21 (pre-pubertal), 43 (onset of puberty), 52 (fully pubertal), 70 (mature), and 116 (fully adult). Serum and intratesticular testosterone and pituitary bioactive chorionic gonadotropin (bioCG) were measured. Testicular development was assessed by ultrasonography, histology, and flow cytometry. Three consecutive blood samples revealed extreme fluctuations in testosterone concentrations, suggesting an erratic secretion. Age-related changes in serum testosterone and pituitary bioCG concentrations were observed. Intratesticular androgens (ITAs) showed high fluctuations within groups at all ages and were high in some animals by 21 wk. Unexpectedly, no correlation between pituitary bioCG and serum testosterone or ITAs was found, but these parameters significantly correlated with testicular weight and volume. These observations were consistent a dependence on the testis growth on bioCG. Unfortunately, the low serum levels of bioCG were not measurable in this study. At 43 wk, the animals reached puberty. At 52 wk of age, animals attained maximum body and epididymal weights and qualitatively normal spermatogenesis, but testes continued growing, reaching a maximum of all parameters at 70 wk of age, without further major changes at the age of 116 wk. It is concluded that (1) gonadal activation is evident at wk 21, (2) the male marmoset reaches the pubertal threshold around 43 wk of age, attains qualitative parameters at 52 wk, matures further to sexual maturity at 70 wk, and (3) serum testosterone and ITAs are highly variable without any identifiable correlation with pituitary bioCG.